Team:Stockholm/20 August 2010
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Revision as of 19:36, 20 August 2010
Contents |
Hassan
midnight update, after a fast recovery from sickness!
Version 0.5.1
Version 0.6.1
Mimmi
pMA
Primer dilution
pMA_VF | 91µl sH2O --> 100µM | 10µl + 90µl sH2O --> 10µM | ||
pMA_VR | 91µl sH2O --> 100µM | 10µl + 90µl sH2O --> 10µM |
pMA/pEX
verification PCR
pEX | pMA | ||||||||
---|---|---|---|---|---|---|---|---|---|
Mix | (µl) | X6 | X3 | primers | conditions | ||||
sH2O | 22.5 | 135 | 67.5 | pEX_VF | time | °C | |||
F primer | 1 | 6 | 3 | pEX_VR | 2m | 94 | |||
R primer | 1 | 6 | 3 | pMA_VF | 30s | 94 | ) | ||
DNA | 0.5 | 5X0.5 | 2X0.5 | pMA_VR | 30s | 60 | > 30 cycles | ||
tot | 25µl | 250µl | 75µl | 2m30s | 72 | ) | |||
10m | 72 | ||||||||
oo | 10 |