Team:Stockholm/20 August 2010

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Revision as of 19:36, 20 August 2010

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Contents


Hassan

midnight update, after a fast recovery from sickness!

Version 0.5.1

Version 0.6.1

Mimmi

pMA

Primer dilution

pMA_VF 91µl sH2O --> 100µM 10µl + 90µl sH2O --> 10µM
pMA_VR 91µl sH2O --> 100µM 10µl + 90µl sH2O --> 10µM


pMA/pEX

verification PCR

pEX pMA
Mix (µl) X6 X3 primers conditions
sH2O 22.5 135 67.5 pEX_VF time °C
F primer 1 6 3 pEX_VR 2m 94
R primer 1 6 3 pMA_VF 30s 94 )
DNA 0.5 5X0.5 2X0.5 pMA_VR 30s 60 > 30 cycles
tot 25µl 250µl 75µl 2m30s 72 )
10m 72
oo 10
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