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Team:LMU-Munich/Schedule
From 2010.igem.org
(→PCR and mutagenisis of the digestion sites) |
(→PCR and mutagenisis of the digestion sites) |
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[[Image:PCR1aP.jpg]] | [[Image:PCR1aP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]][[Team:LMU-Munich/Schedule/mpduD_rev| mpduD_rev]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/mpduD_rev| mpduD_rev]] expected product size: bp |
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[[Image:PCR1bP.jpg]] | [[Image:PCR1bP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/mpduD_fwd| mpduD fwd]][[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/mpduD_fwd| mpduD fwd]],[[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size: bp |
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[[Image:PCR2P.jpg]] | [[Image:PCR2P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]][[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size: bp |
| Line 23: | Line 23: | ||
[[Image:PCR3aP.jpg]] | [[Image:PCR3aP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/mpduC_rev| mpduC rev]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/mpduC_rev| mpduC rev]]expected product size: bp |
| Line 29: | Line 29: | ||
[[Image:PCR3bP.jpg]] | [[Image:PCR3bP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/mpduC_fwd| mpduC fwd]][[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/mpduC_fwd| mpduC fwd]],[[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size: bp |
| Line 35: | Line 35: | ||
[[Image:PCR4P.jpg]] | [[Image:PCR4P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size: bp |
| Line 41: | Line 41: | ||
[[Image:PCR5P.jpg]] | [[Image:PCR5P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/5P3AD| 5P3AD]][[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/5P3AD| 5P3AD]],[[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size: bp |
| Line 47: | Line 47: | ||
[[Image:PCR6aP.jpg]] | [[Image:PCR6aP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/m1lctO_rev| m1lctO rev]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/m1lctO_rev| m1lctO rev]] expected product size: bp |
| Line 53: | Line 53: | ||
[[Image:PCR6bP.jpg]] | [[Image:PCR6bP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/m1lctO_fwd| m1lctO fwd]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/m1lctO_fwd| m1lctO fwd]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size: bp |
| Line 59: | Line 59: | ||
[[Image: PCR7P.jpg]] | [[Image: PCR7P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size: bp |
| Line 65: | Line 65: | ||
[[Image:PCR8aP.jpg]] | [[Image:PCR8aP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/m2lctO_rev| m2lctO rev]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/m2lctO_rev| m2lctO rev]] expected product size: bp |
| Line 71: | Line 71: | ||
[[Image:PCR8bP.jpg]] | [[Image:PCR8bP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/m2lctO_fwd| m2lctO fwd]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/m2lctO_fwd| m2lctO fwd]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size: bp |
| Line 77: | Line 77: | ||
[[Image:PCR9P.jpg]] | [[Image:PCR9P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size: bp |
| Line 83: | Line 83: | ||
[[Image:PCR10P.jpg]] | [[Image:PCR10P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]][[Team:LMU-Munich/Schedule/9P4A| 9P4A]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/9P4A| 9P4A]]expected product size: bp |
| Line 89: | Line 89: | ||
[[Image:PCR11P.jpg]] | [[Image:PCR11P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size: bp |
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[[Image:PCR12aP.jpg]] | [[Image:PCR12aP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/mpduA_rev| mpduA rev]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/mpduA_rev| mpduA rev]] expected product size: bp |
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| - | Primer used:[[Team:LMU-Munich/Schedule/mpduA_fwd| mpduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/mpduA_fwd| mpduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size: bp |
| Line 108: | Line 108: | ||
[[Image:PCR131517P.jpg]] | [[Image:PCR131517P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp |
| Line 114: | Line 114: | ||
[[Image:PCR14aP.jpg]] | [[Image:PCR14aP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/mpduB_rev|mpduB rev]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/mpduB_rev|mpduB rev]]expected product size: bp |
| Line 120: | Line 120: | ||
[[Image:PCR14bP.jpg]] | [[Image:PCR14bP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/mpduB_fwd| mpduB fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/mpduB_fwd| mpduB fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp |
| Line 126: | Line 126: | ||
[[Image:PCR131517P.jpg]] | [[Image:PCR131517P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp |
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[[Image:PCR16aP.jpg]] | [[Image:PCR16aP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]][[Team:LMU-Munich/Schedule/mpduJ_rev|mpduJ rev]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/mpduJ_rev|mpduJ rev]]expected product size: bp |
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[[Image:PCR16bP.jpg]] | [[Image:PCR16bP.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/mpduJ_fwd|mpduJ fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/mpduJ_fwd|mpduJ fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp |
| Line 144: | Line 144: | ||
[[Image:PCR131517P.jpg]] | [[Image:PCR131517P.jpg]] | ||
| - | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp | + | Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size: bp |
==In Vitro testing of the chemical kinetics with the enzymes== | ==In Vitro testing of the chemical kinetics with the enzymes== | ||
Revision as of 13:00, 20 August 2010
PCR1a: replication of Pdu D with mutagenesis [ ]
Primer used: 1P1D, mpduD_rev expected product size: bp
Primer used: mpduD fwd, 3P2D expected product size: bp
Primer used: 1P1D, 3P2D expected product size: bp
Primer used: 2P1C, mpduC revexpected product size: bp
Primer used: mpduC fwd, 4P2C expected product size: bp
Primer used: 2P1C, 4P2C expected product size: bp
Primer used: 5P3AD, 9P4A expected product size: bp
Primer used: 8P3LC, m1lctO rev expected product size: bp
Primer used: m1lctO fwd, 10P4L expected product size: bp
Primer used: 8P3LC, 10P4Lexpected product size: bp
Primer used: 8P3LC, m2lctO rev expected product size: bp
Primer used: m2lctO fwd, 10P4Lexpected product size: bp
Primer used: 8P3LC, 10P4Lexpected product size: bp
Primer used: 1P1D, 9P4Aexpected product size: bp
Primer used: 2P1C, 10P4L expected product size: bp
Primer used: pduA fwd, mpduA rev expected product size: bp
Primer used: pduA fwd,pduU revexpected product size: bp
Primer used: pduA fwd,mpduB revexpected product size: bp
Primer used: mpduB fwd,pduU revexpected product size: bp
Primer used:pduA fwd,pduU revexpected product size: bp
Primer used:pduA fwd,mpduJ revexpected product size: bp
Primer used:mpduJ fwd,pduU revexpected product size: bp
Primer used:pduA fwd,pduU revexpected product size: bp
Contents
PCR and mutagenisis of the digestion sites
PCR1b: replication of Pdu D with mutagenesis [ ]
PCR2: Joining PCR of PduD [ ]
PCR3a: replication of Pdu C with mutagenesis [ ]
PCR3b: replication of Pdu C with mutagenesis [ ]
PCR4: Joining PCR of Pdu C [ ]
PCR5: replication of AurF to be joined with Pdu D [ ]
PCR6a: replication of LctO with mutagenesis [ ]
PCR6b: replication of LctO with mutagenesis [ ]
PCR7: Joining PCR of LctO [ ]
PCR8a: replication of LctO with mutagenesis [ ]
PCR8b: replication of LctO with mutagenesis [ ]
PCR9: Joining PCR of LctO [ ]
PCR10: Joining PCR of AurF and PduD [ ]
PCR11: Joining PCR of LctO and PduC [ ]
PCR12a: replication of the Pdu-Operon with mutagenesis [ ]
PCR12b: replication of the Pdu-Operon with mutagenesis [ ]
Primer used: mpduA fwd,pduU rev expected product size: bp
PCR13: Joining PCR of the Pdu-Operon [ ]
PCR14a: replication of the Pdu-Operon with mutagenesis [ ]
PCR14b: replication of the Pdu-Operon with mutagenesis [ ]
PCR15: Joining PCR of the Pdu-Operon [ ]
PCR16a: replication of the Pdu-Operon with mutagenesis [ ]
PCR16b: replication of the Pdu-Operon with mutagenesis [ ]
PCR17: Joining PCR of the Pdu-Operon [ ]
In Vitro testing of the chemical kinetics with the enzymes
Transforming the construct in bacteria
Testing the system
