Team:LMU-Munich/Cut'N'survive/Schedule
From 2010.igem.org
(→Aim 1: DNA replication+PCR) |
(→Aim 2: Assembling Biobricks) |
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==Aim 2: Assembling Biobricks== | ==Aim 2: Assembling Biobricks== | ||
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+ | We are using the 3A System to assemble Biobricks. | ||
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+ | <b> Assembling Construct 1 </b> | ||
+ | [[Image: N1.bmp]] | ||
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==Aim 3: Testing products== | ==Aim 3: Testing products== | ||
==Aim 4: Testing system== | ==Aim 4: Testing system== |
Revision as of 15:56, 20 August 2010
For PCR key see PCR key
Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer
Primer used: 1TreF 2TreR
expected product size: 492bp
Primer used: 3TevF 4TevMutEPR
expected product size: 332 bp
Primer used: 5TevMutEPF 6TevR
expected product size: 772 bp
Primer used: 3TevF 6TevR
expected product size: 1087 bp
Primer used: 7BakF 8BakMutPR
expected product size: 330 bp
Primer used: 9BakMutPF 10BakR
expected product size: 376 bp
Primer used: 7BakF 10BakR
expected product size: 688 bp
Primer used: 11PAF 12PAR
expected product size: 237 bp
Primer used: 13TrecF 14TrecMutSR
expected product size: 850 bp
Primer used: 15TrecMutSF 16TrecR
expected product size: 402 bp
Primer used: 13TrecF 16TrecR
expected product size: 1233 bp
We are using the 3A System to assemble Biobricks.
Assembling Construct 1
File:N1.bmp
Contents
Aim 1: DNA replication+PCR
PCR1: replication of the Tet-inducible CMV promotor [X]
PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]
PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]
PCR3: joining PCR of the TEVrecogn-NDegron-SF3 Part [ ]
PCR4a: replication human bak with mutagenisis (Pst1) [ ]
PCR4b: replication human bak with mutagenisis (Pst1) [ ]
PCR5: joining PCR of human bak [ ]
PCR6: replication of the SV40-polyadenylation site [X]
PCR7a: replication of the p14*TEVrecogn with mutagenisis (Spe1) [ ]
PCR7b: replication of the p14*TEVrecogn with mutagenisis (Spe1) with TEV recogn in primer (16TrecR) [ ]
PCR8: joining PCR of p14*TEVrecogn with TEV recogn in primer (16TrecR) [ ]
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system