Team:LMU-Munich/Jump-or-die/Schedule

(Difference between revisions)

Revision as of 11:51, 20 August 2010


	Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule

	Functional Principle Sequences Schedule picture gallery

For PCR key see PCR key

Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer

PCR1: replication of the Tet-inducible CMV promotor [X]

Primer used: 1TreF 2TreR expected product size: 492 bp

PCR4a: replication human bak with mutagenesis (Pst1) [ ]

Primer used: 7BakF 8BakMutPR expected product size: 330 bp

PCR4b: replication human bak with mutagenesis (Pst1) [ ]

Primer used: 9BakMutPF 10BakR expected product size: 376 bp

PCR5: joining PCR of human bak [ ]

Primer used: 7BakF 10BakR expected product size: 688 bp

PCR6: replication of the SV40-polyadenylation site [X]

Primer used: 11PAF 12PAR expected product size: 237 bp

PCR9: replication of eGFP with attP in primer [ ]

Primer used: 20eGFPattPF 21eGFPR expected product size: 808 bp

PCR10: Replikation of PhiC31o [ ]

Primer used: 22PC31oF 23PC31oR expected product size: 1888 bp

Note: for attB two Primers ( 18attBF 19attBR) used.

@@ Line 47: / Line 47: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer20| 20eGFPattPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer21| 21eGFPR]]
-expected product size: 1213 bp
+expected product size: 808 bp
@@ Line 54: / Line 54: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer22| 22PC31oF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer23| 23PC31oR]]
-expected product size:  bp
+expected product size: 1888 bp
 <b>Note: for attB two Primers ([[Team:LMU-Munich/Cut'N'survive/Schedule/Primer18| 18attBF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer19| 19attBR]]) used.</b>