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Filamentous Cells
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| Wild type Bacillus subtillis [[Image:Wild type Bacillus subtillis.jpg]] | | Wild type Bacillus subtillis [[Image:Wild type Bacillus subtillis.jpg]] | ||
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| - | |dinR KO [[Image:dinR KO]] | + | |dinR KO [[Image:dinR KO.jpg]] |
|- | |- | ||
| - | |dinR KO mutant over expressed the divergent (opposite direction) transcript for YneA, YneB and YnzC. These genes form the SOS regulon (recA independent SOS response). [[Image:Coding region]] | + | |dinR KO mutant over expressed the divergent (opposite direction) transcript for YneA, YneB and YnzC. These genes form the SOS regulon (recA independent SOS response). [[Image:Coding region.jpg]] |
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|YneA suppressed in wt without SOS induction | |YneA suppressed in wt without SOS induction | ||
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|Disruption of YneA in SOS response leads to reduced elongation. Altering YneB and YnzC expression does not affect cell morphology. | |Disruption of YneA in SOS response leads to reduced elongation. Altering YneB and YnzC expression does not affect cell morphology. | ||
|- | |- | ||
| - | |Double mutant (dinR/YneA) [[Image:Double Mutant]] | + | |Double mutant (dinR/YneA) [[Image:Double Mutant.jpg]] |
|- | |- | ||
|YneA protein required to suppress cell division. Not chromosome replication or segregation. | |YneA protein required to suppress cell division. Not chromosome replication or segregation. | ||
Revision as of 12:25, 29 May 2010
Filamentous Cells
| SOS response is believe to be a universal bacteria phenomenon first studied in E.coli -LexA, recA |
| In Bacillus subtillis (gram positive) dinR protein is homologous to lexA (Repressor of din-damage inducible genes).din genes include uvrA, uvrB, dinB, dinC dinR and recA. DNA damage inhibits cell division. |
Wild type Bacillus subtillis 
|
dinR KO 
|
dinR KO mutant over expressed the divergent (opposite direction) transcript for YneA, YneB and YnzC. These genes form the SOS regulon (recA independent SOS response). 
|
| YneA suppressed in wt without SOS induction |
| Expression of YneA from ITPG controlled promoter in wt leads to elongation. |
| Disruption of YneA in SOS response leads to reduced elongation. Altering YneB and YnzC expression does not affect cell morphology. |
Double mutant (dinR/YneA) 
|
| YneA protein required to suppress cell division. Not chromosome replication or segregation. |
| FtsZ is important for bacterial cell division forming a ring structure at the division site by polymerising
assembling other proteins necessary for division at the site. |
| FtsZ localises to the cell division cycle unless dinR is disrupted or YneA is being induced. |
| YneA suppresses FtsZ ring formation- no proven direct interaction by two-hybrid. |
| Filamentous cells less colony formation. |
| YneA expression via the inactivation of dinR by Rec A is important. |
| (Kawai et al. 2003) |
