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Team:LMU-Munich/Schedule
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{{:Team:LMU-Munich/Templates/Page Header}} | {{:Team:LMU-Munich/Templates/Page Header}} | ||
==PCR and mutagenisis of the digestion sites== | ==PCR and mutagenisis of the digestion sites== | ||
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| + | PCR1a: replication of Pdu D with mutagenisis | ||
| + | [[Image:PCR1aP]] | ||
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| + | PCR1b: replication of Pdu D with mutagenisis | ||
| + | [[Image:PCR1bP]] | ||
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| + | PCR2: Joining PCR of PduD | ||
| + | [[Image:PCR2P]] | ||
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| + | PCR3a: replication of Pdu C with mutagenisis | ||
| + | [[Image:PCR3aP]] | ||
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| + | PCR3b: replication of Pdu C with mutagenisis | ||
| + | [[Image:PCR3bP]] | ||
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| + | PCR4: Joining PCR of Pdu C | ||
| + | [[Image:PCR4P]] | ||
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| + | PCR5: replication of AurF to be joined with Pdu D | ||
| + | [[Image:PCR5P]] | ||
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| + | PCR6a: replication of LctO with mutagenisis | ||
| + | [[Image:PCR6aP]] | ||
| + | |||
| + | PCR6b: replication of LctO with mutagenisis | ||
| + | [[Image:PCR6bP]] | ||
| + | |||
| + | PCR7: Joining PCR of LctO | ||
| + | [[Image: PCR7P]] | ||
| + | |||
| + | PCR8a: replication of LctO with mutagenisis | ||
| + | [[Image:PCR8aP]] | ||
| + | |||
| + | PCR8b: replication of LctO with mutagenisis | ||
| + | [[Image:PCR8bP]] | ||
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| + | PCR9: Joining PCR of LctO | ||
| + | [[Image:PCR9P]] | ||
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| + | PCR10: Joining PCR of AurF and PduD | ||
| + | [[Image:PCR10P]] | ||
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| + | PCR11: Joining PCR of LctO and PduC | ||
| + | [[Image:PCR11P]] | ||
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| + | PCR12a: replication of the Pdu-Operon with mutagenisis | ||
| + | [[Image:PCR12aP]] | ||
| + | |||
| + | PCR12b: replication of the Pdu-Operon with mutagenisis | ||
| + | [[Image:PCR12bP]] | ||
| + | |||
| + | PCR13: Joining PCR of the Pdu-Operon | ||
| + | [[Image:PCR131517P]] | ||
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| + | PCR14a: replication of the Pdu-Operon with mutagenisis | ||
| + | [[Image:PCR14aP]] | ||
| + | |||
| + | PCR14b: replication of the Pdu-Operon with mutagenisis | ||
| + | [[Image:PCR14bP]] | ||
| + | |||
| + | PCR15: Joining PCR of the Pdu-Operon | ||
| + | [[Image:PCR131517P]] | ||
| + | |||
| + | PCR16a: replication of the Pdu-Operon with mutagenisis | ||
| + | [[Image:PCR16aP]] | ||
| + | |||
| + | PCR16b: replication of the Pdu-Operon with mutagenisis | ||
| + | [[Image:PCR16bP]] | ||
| + | |||
| + | PCR17: Joining PCR of the Pdu-Operon | ||
| + | [[Image:PCR131517P]] | ||
| + | |||
==In Vitro testing of the chemical kinetics with the enzymes== | ==In Vitro testing of the chemical kinetics with the enzymes== | ||
==Transforming the construct in bacteria== | ==Transforming the construct in bacteria== | ||
==Testing the system== | ==Testing the system== | ||
{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} | ||
Revision as of 11:05, 20 August 2010
PCR1a: replication of Pdu D with mutagenisis
File:PCR1aP
PCR1b: replication of Pdu D with mutagenisis
File:PCR1bP
PCR2: Joining PCR of PduD
File:PCR2P
PCR3a: replication of Pdu C with mutagenisis
File:PCR3aP
PCR3b: replication of Pdu C with mutagenisis
File:PCR3bP
PCR4: Joining PCR of Pdu C
File:PCR4P
PCR5: replication of AurF to be joined with Pdu D
File:PCR5P
PCR6a: replication of LctO with mutagenisis
File:PCR6aP
PCR6b: replication of LctO with mutagenisis
File:PCR6bP
PCR7: Joining PCR of LctO
File:PCR7P
PCR8a: replication of LctO with mutagenisis
File:PCR8aP
PCR8b: replication of LctO with mutagenisis
File:PCR8bP
PCR9: Joining PCR of LctO
File:PCR9P
PCR10: Joining PCR of AurF and PduD
File:PCR10P
PCR11: Joining PCR of LctO and PduC
File:PCR11P
PCR12a: replication of the Pdu-Operon with mutagenisis
File:PCR12aP
PCR12b: replication of the Pdu-Operon with mutagenisis
File:PCR12bP
PCR13: Joining PCR of the Pdu-Operon
File:PCR131517P
PCR14a: replication of the Pdu-Operon with mutagenisis
File:PCR14aP
PCR14b: replication of the Pdu-Operon with mutagenisis
File:PCR14bP
PCR15: Joining PCR of the Pdu-Operon
File:PCR131517P
PCR16a: replication of the Pdu-Operon with mutagenisis
File:PCR16aP
PCR16b: replication of the Pdu-Operon with mutagenisis
File:PCR16bP
PCR17: Joining PCR of the Pdu-Operon
File:PCR131517P
Contents
PCR and mutagenisis of the digestion sites
In Vitro testing of the chemical kinetics with the enzymes
Transforming the construct in bacteria
Testing the system
