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Team:Stockholm/19 August 2010
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====Sequencing results==== | ====Sequencing results==== | ||
| - | Received the sequencing results from Eurofins. Ran a nucleotide BLAST (BLASTn) alignment to verify successful site-directed mutagenesis. | + | Received the sequencing results for [[media:M-sod1_premix_19aug.txt|m-SOD 1]], [[media:M-sod2_premix_19aug.txt|m-SOD 2]], [[media:M-yCCS1_premix_19aug.txt|m-yCCS 1]] and [[media:M-yCCS4_premix_19aug.txt|m-yCCS 4]] from Eurofins. Ran a nucleotide BLAST (BLASTn) alignment to verify successful site-directed mutagenesis. |
*'''SOD 1:''' Failed | *'''SOD 1:''' Failed | ||
**Mutation still present | **Mutation still present | ||
Revision as of 09:58, 19 August 2010
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Andreas
Site-directed mutagenesis of SOD & yCCS
Sequencing results
Received the sequencing results for m-SOD 1, m-SOD 2, m-yCCS 1 and m-yCCS 4 from Eurofins. Ran a nucleotide BLAST (BLASTn) alignment to verify successful site-directed mutagenesis.
- SOD 1: Failed
- Mutation still present
- SOD 2: Success
- Mutation removed
- yCCS 1: Success
- EcoRI site removed
- yCCS 4: Success
- EcoRI site removed
SOD 2 sample and glycerol stock will be saved, while SOD 1 will be discarded.
Unfortunately, BLAST results also discovered 4 (!) identical mutations in both yCCS sequences. A protein BLAST (BLASTp) alignment of the translated yCCS sequences revealed all four mutations as silent mutations.
