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AUGUST: WEEK 3
August, 16th
Resuspension from iGEM 2010 Distribution Kit of <partinfo>BBa_J13002</partinfo> (Plate 1, Well 13B) and <partinfo>BBa_I13522</partinfo> (Plate 2, Well 8A).
Transformation (1 ul) of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM (Mr.Gene) into 100 ul of E. coli TOP10 home-made competent cells.
Transformation of 15-4C5 (1 ul) into in re-competentized E. coli TOP10 carrying <partinfo>BBa_T9002</partinfo>.
Transfomants were plated on LB+Amp 100 ug/ml agar plates, the last one on LB+Amp100+Cm12,5 agar plates and were let grow ON at 37°C.
August, 17th
All plates showed colonies.
Pick of <partinfo>BBa_J13002</partinfo>, <partinfo>BBa_I13522</partinfo>, INTEIN, CREAM, co-trasformed <partinfo>BBa_T9002</partinfo> and inoculum into 1 ml of LB+Amp, LB+Kan, LB+Cm, respectively, to make glycerol stocks. Falcons were than refilled for the minipreps of the following day.
Cultures were diluted (5ul in 2ml LB+antibiotic) for TECAN test:
| I7-A | I8-5 D | I9-1
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| I10-B | I12-2 | A2
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| <partinfo>BBa_B0032</partinfo> | I144C5-T9002 | I164C5-T9002
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| I174C5-T9002 | I184C5-T9002 | I194C5-T9002
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| ENTERO4C5-T9002 | |
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Glycerol stock was prepared for ENTERO4C5-T9002.
August, 18th
Cultures were diluted (5ul in 2ml LB+antibiotic) for TECAN test:
| I7-A | I8-5 D | I9-1
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| I10-B | I12-2 | A2
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| <partinfo>BBa_B0032</partinfo> | I144C5-T9002 | I154C5-T9002
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| I164C5-T9002 | I174C5-T9002 | I184C5-T9002
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| I194C5-T9002 | ENTERO4C5-T9002 | |
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Glycerol stock was prepared for I154C5-T9002.
August, 19th
August, 20th
August, 21st
August, 22nd
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