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| | Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/ here (www.igem-ut.net/blog) ]. | | Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/ here (www.igem-ut.net/blog) ]. |
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| - | == '''Protocol''' ==
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| - |
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| - | === Transformation ===
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| - |
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| - | -preparation
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| - | ・iGEM parts / ligation products
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| - | ・LBbroth (No antibiotic) 500uL
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| - | ・TE 15uL
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| - | ・competent cells
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| - | ・plates
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| - | ・ice box
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| - | ・heat block(42℃)
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| - |
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| - | -Notice
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| - | competent cells
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| - | → always onice!!! Melt on ice! Mix DNA as soon as cells melt!
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| - |
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| - | -Protocol
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| - | iGEMパーツを解凍する場合
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| - | ↓P200のピペットマンにチップを付け、チップでフィルムに孔を空ける。
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| - | ↓add 15µl TE（MilliQで可）、ピペッティング
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| - | ↓1µlをコンピテントセルにいれ30min on ice(コンピに対し1/5倍量以下になるように)
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| - | iGEM parts
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| - | Hole film with a tip
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| - | ↓Add 15 uL TE or MilliQ, pipetting
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| - | ↓Mix 1uL with competent cells 50uL
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| - | ↓on ice 30 min
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| - | ↓42℃ 45 sec
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| - | ↓on ice 10 min
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| - | ↓Mix LBbroth 300 uL
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| - | ↓37℃ 30 min or room temprature 15 min (amp:omittable)
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| - | ↓Plate, 37℃ incubation over night
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| - | Ligation products
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| - | ↓Mix ligation products 10uL, competent cells 50uL
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| - |
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| - | ↓on ice 30 min
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| - |
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| - | ↓42℃ 45 sec
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| - |
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| - | ↓on ice 10 min
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| - |
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| - | ↓Mix LBbroth 300 uL
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| - |
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| - | ↓37℃ 30 min or room temprature 15 min (amp:omittable)
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| - |
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| - | ↓Plate, 37℃ incubation over night
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| - |
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| | | | |
| | </div> | | </div> |
| | {{UT-Tokyo_FOOTER}} | | {{UT-Tokyo_FOOTER}} |
Notebook
Our experiment notebooks (Japanese) can be read [http://www.igem-ut.net/blog/ here (www.igem-ut.net/blog) ].
"
