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Team:LMU-Munich/Jump-or-die/Schedule
From 2010.igem.org
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(→Aim 1: DNA reproduction+PCR) |
(→Aim 1: DNA reproduction+PCR) |
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Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]] | ||
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| + | PCR9: replication of eGFP with attP in primer | ||
| + | [[Image:PCR9.jpg]] | ||
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| + | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer20| 20eGFPattPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer21| 21eGFPR]] | ||
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| + | PCR10: Replikation of PhiC31o | ||
| + | [[Image:PCR10.jpg]] | ||
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| + | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer22| 22PC31oF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer23| 23PC31oR]] | ||
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| + | Note: for attB two Primers ([[Team:LMU-Munich/Cut'N'survive/Schedule/Primer18| 18attBF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer19| 19attBR]]) used. | ||
==Aim 2: Assembling Biobricks== | ==Aim 2: Assembling Biobricks== | ||
Revision as of 13:17, 18 August 2010
PCR1: replication of the Tet-inducible CMV promotor [X]
PCR4a: replication human bak with mutagenisis (Pst1) [ ]
PCR4b: replication human bak with mutagenisis (Pst1) [ ]
PCR5: joining PCR of human bak [ ]
PCR6: replication of the SV40-polyadenylation site [X]
PCR9: replication of eGFP with attP in primer
Primer used: 20eGFPattPF 21eGFPR
PCR10: Replikation of PhiC31o
Primer used: 22PC31oF 23PC31oR
Note: for attB two Primers ( 18attBF 19attBR) used.
Contents
Aim 1: DNA reproduction+PCR
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
