Team:Michigan/Pili Expression
From 2010.igem.org
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*incubate CIP-pBAD at 37C for 1 hr and then heat-shock (65C for 15 min) | *incubate CIP-pBAD at 37C for 1 hr and then heat-shock (65C for 15 min) | ||
- | '''We made a huge human error when setting up our thermal cycle program on the PCR machine. We did not realize we only set the thermal cycle for our digest on [ | + | '''We made a huge human error when setting up our thermal cycle program on the PCR machine. We did not realize we only set the thermal cycle for our digest on [https://2010.igem.org/Team:Michigan/Pili_Expression#8.2F14.2F2010] for 12 minutes (12:00) rather than the intended time period of 12 hours (12:00:00). This explains the condensation present in the PCR machine (it stayed at 4C for too long). We discovered our mistake after running the incubation of CIP+pBAD for 37C for (1:00) 1 minute then heat-shock at 65C for 15 minute. It is VERY IMPORTANT to triple check one another when entering in any program.''' |
Rest of the day: | Rest of the day: |
Revision as of 05:06, 17 August 2010