Team:Michigan/Pili Expression
From 2010.igem.org
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==8/14/2010== | ==8/14/2010== | ||
''Kevin, Marc, Alena'' | ''Kevin, Marc, Alena'' | ||
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'''Miniprep pBAD plasmid''' | '''Miniprep pBAD plasmid''' | ||
#inoculate 5.0mL LB in 50mL conical tubes w/ 100ug/mL of ampilicin | #inoculate 5.0mL LB in 50mL conical tubes w/ 100ug/mL of ampilicin | ||
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**2 uL of NEB 2 buffer | **2 uL of NEB 2 buffer | ||
**1 uL NcoI | **1 uL NcoI | ||
+ | **1uL HindIII | ||
#incubate for 37C overnight (12hrs) | #incubate for 37C overnight (12hrs) | ||
##place into 4C fridge the next day | ##place into 4C fridge the next day | ||
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Revision as of 08:15, 16 August 2010