Team:Osaka/notebooktest

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Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura
Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura
# Safety lecture for junior members.
# Safety lecture for junior members.
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# Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).</div>
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# Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).
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<div class="CollapsiblePanelTab">July 31 (Thu)</div>
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Attendance: Miyatake, Hirayama, Torigata, Teoh, Tadashi, Yasumoto, Kakuda, Saka
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# Meeting
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#* Summer project schedule
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#* List of genes to clone</div>
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<div class="CollapsiblePanelTab">August 2 (Mon)</div>
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Attendance: Torigata, Takino, Teoh, Tadashi, Yasumoto, Saka
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# Culture medium preparation
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#* LB agar plates (49 antibiotic-less plates)
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#* LB liquid medium (500 ml)
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# Competent cells preparation - Nojima Method
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#* SOB medium (MgCl2 not yet added) -> stored at 4˚C
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#* TB buffer -> stored at 4˚C
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#* Single-colony streaking of E. coli (strain DH5α) on 2 LB agar plates -> 37˚C incubation o/n
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Tomorrow we shall prepare the remainder of the reagents and inoculate SOB with o/n-incubated E. coli.</div>
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Latest revision as of 14:46, 15 August 2010

July 29 (Thu)
Attendance: Torigata, Takino, Teoh, Yasumoto, Kakuda, Saka, Tamura # Safety lecture for junior members. # Preparation of LB agar plates (26 Amp, 25 Kan, 25 Cam).

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