Team:Harvard/vectors/vectors
From 2010.igem.org
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<p>In our second scheme, we will knock out BETA-OHASE 1 on its own. We hope that this will induce accumulation of beta-carotene.</p><br/><br/> | <p>In our second scheme, we will knock out BETA-OHASE 1 on its own. We hope that this will induce accumulation of beta-carotene.</p><br/><br/> | ||
<img src="https://static.igem.org/mediawiki/2010/6/6b/Ath00906_carotenoid_2.png" width="550px"> | <img src="https://static.igem.org/mediawiki/2010/6/6b/Ath00906_carotenoid_2.png" width="550px"> | ||
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<p>In our third scheme, we will knock out both LUT2 and BETA-OHASE1. We hope that this will induce accumulation of some lycopene and some beta-carotene.</p><br/> | <p>In our third scheme, we will knock out both LUT2 and BETA-OHASE1. We hope that this will induce accumulation of some lycopene and some beta-carotene.</p><br/> | ||
<img src="https://static.igem.org/mediawiki/2010/5/5e/Ath00906_carotenoid_3.png" width="550px"> | <img src="https://static.igem.org/mediawiki/2010/5/5e/Ath00906_carotenoid_3.png" width="550px"> |
Revision as of 16:12, 11 August 2010
![](https://static.igem.org/mediawiki/2010/f/fd/Harvard2010vectorbannercrop.png)
meet the vectors
Our vectors are a modification of the pORE agrobacterium vector system developed by Coutu et al at Agriculture and Agrofood Canada. This vector system is designed for agrobacterium mediated plant transformation, and is a binary system with the vector containing the site for foreign constructs and the appropriate flanking regions to be recognized for introduction to the plant. The virulence genes are present on a separate vector contained in the agrobacterium, separate from the TDNA.
To modify the pORE series binary vectors to fit the biobrick standard, we digested the vectors with. We worked with two of each of the open, reporter, and expression series vectors, creating a total of six biobrick standard vectors suitable for agrobacterium mediated plant transformation.
![](https://static.igem.org/mediawiki/2010/8/87/Open_Small.png)
The open series vectors are designed for general insertion of a construct. We modified the vectors pORE O1 and pORE O2. Each has a different antibiotic resistance.
In our second scheme, we will knock out BETA-OHASE 1 on its own. We hope that this will induce accumulation of beta-carotene.
![](https://static.igem.org/mediawiki/2010/6/6b/Ath00906_carotenoid_2.png)
In our third scheme, we will knock out both LUT2 and BETA-OHASE1. We hope that this will induce accumulation of some lycopene and some beta-carotene.
![](https://static.igem.org/mediawiki/2010/5/5e/Ath00906_carotenoid_3.png)
open series click to enlarge ![]() |
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reporter series click to enlarge ![]() |
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expression series click to enlarge ![]() |
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