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Team:Newcastle/DNA re-hydration
From 2010.igem.org
(Difference between revisions)
(New page: This protocol is used for the re-hydration of DNA. We mainly used this for our newly arrived primers. (Link) =Materials required= * Oligonucleotide Specification Sheet * OD 260 values * ...) |
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* Calculator (!) | * Calculator (!) | ||
| - | = | + | =Methods= |
# To begin, read the Oligonucleotide Specification Sheet to find the OD 260 values and Quantity of DNA bases for each primer/DNA | # To begin, read the Oligonucleotide Specification Sheet to find the OD 260 values and Quantity of DNA bases for each primer/DNA | ||
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# Multiple the result by 1000 | # Multiple the result by 1000 | ||
# Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in microlitres. | # Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in microlitres. | ||
| - | # Add Pure Lab Distilled Water to make the solution 100 microlitres in total. | + | # Add Pure Lab Distilled Water to make the solution up to 100 microlitres in total. |
NOTE: Extra care must be taken when following this protocol, i.e. NO contamination. | NOTE: Extra care must be taken when following this protocol, i.e. NO contamination. | ||
Revision as of 09:06, 11 August 2010
This protocol is used for the re-hydration of DNA. We mainly used this for our newly arrived primers. (Link)
Materials required
- Oligonucleotide Specification Sheet
- OD 260 values
- Quantity of DNA bases
- Pure Lab Distilled Water
- Calculator (!)
Methods
- To begin, read the Oligonucleotide Specification Sheet to find the OD 260 values and Quantity of DNA bases for each primer/DNA
- Once those values are found, divide the (OD 260 value) by (Quantity of DNA bases x10).
- Multiple the result by 1000
- Divide 10 by the previous result, and multiple all by 100: the end result is the volume of primer/DNA in microlitres.
- Add Pure Lab Distilled Water to make the solution up to 100 microlitres in total.
NOTE: Extra care must be taken when following this protocol, i.e. NO contamination.
