Team:UNIPV-Pavia/Calendar/August/settimana2
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- | Transformation of 100 ul of MG008 and MC123 competent | + | Transformation of 100 ul of MG008 and MC123 competent cells to check their efficiency. We used 1 ul (~4 ng) of RING, F2620-4C5, Nothing (water) and plated on proper LB agar plates: |
*RING: Cm 34 ug/ml | *RING: Cm 34 ug/ml | ||
- | *F2620-4C5: Cm 12,5 ug/ml | + | *F2620-4C5 (positive control): Cm 12,5 ug/ml |
- | *Nothing: Cm 12,5 ug/ml | + | *Nothing (negative control): Cm 12,5 ug/ml |
+ | Both RING and F2620-4C5 should survive, but we had a problem so that MC008 transformed with RING couldn't be plated. We will check it another time. | ||
==August, 10th== | ==August, 10th== |
Revision as of 18:57, 9 August 2010
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