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Team:Michigan/Protocols
From 2010.igem.org
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| - | [[Media:7-28-2010_Biofilm_Formation_Experiment.pdf|biofilm assay protocol]] | + | [[Media:7-28-2010_Biofilm_Formation_Experiment.pdf|Revised biofilm assay protocol]] |
| + | *[[Media:7-28-2010_Biofilm_Formation_Experiment.pdf|biofilm assay protocol]] | ||
====Quorum Sensing==== | ====Quorum Sensing==== | ||
Revision as of 18:16, 9 August 2010
Protocols
Using Lab Equipment
Obtaining Deionized Water in the ERB
Enigeering Research Building Autoclave
Epifluorescence Microscope Usage - H.H. Dow
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation-electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
Group-Specific Protocols
Oil Sands
Revised biofilm assay protocol
Quorum Sensing
General Outline for Biobrick Manipulations
