Team:Michigan/Oil Sands
From 2010.igem.org
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'''Bacterial Growth at pH 9''' | '''Bacterial Growth at pH 9''' | ||
- | At 9:00am the two cultures started in the BH-CHCA with the pH actually adjusted to around 9 was checked. Both P. putida oilsands and P. fluorescens oilsands showed slight signs of growth. Later in the day | + | At 9:00am the two cultures started in the BH-CHCA with the pH actually adjusted to around 9 was checked. Both P. putida oilsands and P. fluorescens oilsands showed slight signs of growth. Later in the day at3:30 pm the cultures are denser were denser and the OD600 of the culture is: |
+ | |||
+ | *P. putida oilsands: 0.065 | ||
+ | *P. fluorescens oilsands: 0.433 | ||
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+ | It should be noted some of the salts precipitated out of solution in the 1 mL cultures | ||
'''Miniprep of pBAD take 2''' | '''Miniprep of pBAD take 2''' | ||
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'''Nanodrop of pBAD take 2''' | '''Nanodrop of pBAD take 2''' | ||
- | The concentration of the pBAD promoter miniprepped earlier today is 418 ng/uL | + | The concentration of the pBAD promoter miniprepped earlier today is 418 ng/uL. It is most likely still contaminated with this high concentration but we will digest it and run a gel to check anyways |
'''Digest of pBAD take 2''' | '''Digest of pBAD take 2''' |
Revision as of 15:05, 6 August 2010