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Team:UNIPV-Pavia/Calendar/August/settimana2
From 2010.igem.org
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<html><p align="center"><font size="4"><b>AUGUST: WEEK 2</b></font></p></html><hr><br> | <html><p align="center"><font size="4"><b>AUGUST: WEEK 2</b></font></p></html><hr><br> | ||
==August, 9th== | ==August, 9th== | ||
| + | Phasins plates showed very few colonies (17 for I20-new and 12 for I21-new): they were all picked and let grow in LB+Amp 100ug/ml. In the evening we made glycerol stocks and re-filled the tubes for the screening of the following day: | ||
| + | {|border="1" align="center" | ||
| + | |'''I20-new:'''||I20-1||I20-2||I20-3||I20-4||I20-5||I20-6||I20-7||I20-8||I20-9||I20-10||I20-11||I20-12||I20-13||I20-14||I20-15||I20-16||I20-17 | ||
| + | |- | ||
| + | |'''I21-new:'''||I21-1||I21-2||I21-3||I21-4||I21-5||I21-6||I21-7||I21-8||I21-9||I21-10||I21-11||I21-12 | ||
| + | |} | ||
| + | |||
| + | ---- | ||
| + | Check of LB+Cm 6 ug/ml agar plates. We let grow at 30°C: | ||
| + | *MG1655 in LB | ||
| + | *MC1061 in LB | ||
| + | *MG123/008 in LB+Amp 50 ug/ml | ||
| + | *MC123/008 in LB+Amp 50 ug/ml | ||
| + | Than we streaked cultures on plates. Since 6 ug/ml is a very low concentration, we wanted to check if actually nothing (without the right resistance) grow on these plates. | ||
==August, 10th== | ==August, 10th== | ||
Revision as of 10:30, 9 August 2010
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