Team:UNIPV-Pavia/Material Methods/Instruments
From 2010.igem.org
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=BACTERIA ROOM= | =BACTERIA ROOM= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
This is a room in which we work with bacteria. The temperature in this room is very hot and it smells like puke. | This is a room in which we work with bacteria. The temperature in this room is very hot and it smells like puke. | ||
</p></html> | </p></html> | ||
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_bacteriaroom.jpg | 250px |right|Bacteria room]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=INCUBATOR FOR PLATES= | =INCUBATOR FOR PLATES= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
This is a oven in which plates are kept at 37 C to grow bacteria. | This is a oven in which plates are kept at 37 C to grow bacteria. | ||
- | </p></html> | + | </p></html> |
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_incubatorforplates.jpg | 250px |right|Incubator for plates]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=INCUBATOR AND SHAKER FOR LIQUID CULTURES= | =INCUBATOR AND SHAKER FOR LIQUID CULTURES= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
This is a oven where bacteria are kept to grow. In this case you can define temperature an rpm. | This is a oven where bacteria are kept to grow. In this case you can define temperature an rpm. | ||
- | </p></html> | + | </p></html> |
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_incubatorshaker.jpg | 250px |right|Incubator and shaker for liquid cultures]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=MOLECULAR BIOLOGY ROOM= | =MOLECULAR BIOLOGY ROOM= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
This is the room in which we treat DNA. | This is the room in which we treat DNA. | ||
- | </p></html> | + | </p></html> |
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_molbioroom.jpg | 250px |right|Molecular biology room room]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=HOOD= | =HOOD= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
It is a laminar flow hood for handling highly toxic products. | It is a laminar flow hood for handling highly toxic products. | ||
- | </p></html> | + | </p></html> |
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_hood.jpg | 250px |right|Hood]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=WATER BATH= | =WATER BATH= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
It is a bath in which water is heated to high temperatures for gel extraction and transformation. | It is a bath in which water is heated to high temperatures for gel extraction and transformation. | ||
- | </p></html> | + | </p></html> |
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_waterbath.jpg | 250px |right|Water bath]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
- | = | + | =INSTRUMENTS FOR ELECTROPHORESIS= |
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
Gel electrophoresis apparatus - An agarose gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. | Gel electrophoresis apparatus - An agarose gel is placed in this buffer-filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. | ||
</p></html> | </p></html> | ||
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_electrophoresis.jpg | 250px |right|Instruments for electrophoresis]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=GEL IMAGE ACQUISITION SYSTEM= | =GEL IMAGE ACQUISITION SYSTEM= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
It is a UV system with camera used to acquire gel run snapshots. | It is a UV system with camera used to acquire gel run snapshots. | ||
- | </p></html> | + | </p></html> |
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_gelacq.jpg | 250px |right|Gel image acquisition system]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=FREEZER ROOM= | =FREEZER ROOM= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
This is a room where Dna and bacterial cultures are stocked. In particular we have two -20 C freezers to stock DNA and one -80 C freezer to stock bacteria. | This is a room where Dna and bacterial cultures are stocked. In particular we have two -20 C freezers to stock DNA and one -80 C freezer to stock bacteria. | ||
</p></html> | </p></html> | ||
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_freezer.jpg | 250px |right|Freezer room]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=FRIDGE ROOM= | =FRIDGE ROOM= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
This is a room in which we have a fridge to stock plates and LB medium | This is a room in which we have a fridge to stock plates and LB medium | ||
</p></html> | </p></html> | ||
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_fridge.jpg | 250px |right|Fridge room]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> | ||
=TECAN Infinite 2000= | =TECAN Infinite 2000= | ||
+ | <table><tr><td valign="top"> | ||
<html><p align="justify"> | <html><p align="justify"> | ||
Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates. | Tecan Infinite F200 is a multifunctional microplate filter-based reader with injector option, that provides high performance for the vast majority of microplate applications and research. It has been designed as a general purpose laboratory instrument for professional use, supporting common 6 to 384-well microplates. | ||
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*absorbance | *absorbance | ||
</p></html> | </p></html> | ||
+ | </td> | ||
+ | <td width="20%" valign='top'> | ||
+ | {| border="1" | ||
+ | | [[Image:UNIPV_Pavia_tecan.jpg | 250px |right|TECAN Infinite 2000]] | ||
+ | |} | ||
+ | </td> | ||
+ | </tr> | ||
+ | </table> | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
<br> | <br> |
Revision as of 15:14, 5 August 2010
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