Week of 7/26
From 2010.igem.org
(Difference between revisions)
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+ | ==July 26th== | ||
+ | |||
+ | *Colony PCR: | ||
+ | -J24679+B1002+K3 | ||
+ | -J24679+B1006+K3 | ||
+ | -Lux+RBS+B1006+K3 redo | ||
+ | -Lux+RBS+B1002+K3 | ||
+ | |||
+ | *Digests: | ||
+ | -Lux+RBS (EP) | ||
+ | -A3 (EP+Phosphatase) | ||
+ | |||
+ | *Ligations: | ||
+ | -Lux+RBS+A3 | ||
+ | |||
+ | *Gel Order: | ||
+ | -Ladder | ||
+ | -J24679+B1002+K3 (A-E) | ||
+ | -J24679+B1006+K3 (A-E) | ||
<center>[[Image:7-26-10.jpg]]</center> | <center>[[Image:7-26-10.jpg]]</center> | ||
+ | The bands for 1B and 1D showed up so they were grown up accordingly. | ||
+ | |||
+ | |||
+ | ==July 27th== | ||
+ | |||
+ | *Digests: | ||
+ | - | ||
<center>[[Image:7-27-10.jpg]]</center> | <center>[[Image:7-27-10.jpg]]</center> |
Revision as of 16:01, 5 August 2010
July 26th
- Colony PCR:
-J24679+B1002+K3 -J24679+B1006+K3 -Lux+RBS+B1006+K3 redo -Lux+RBS+B1002+K3
- Digests:
-Lux+RBS (EP) -A3 (EP+Phosphatase)
- Ligations:
-Lux+RBS+A3
- Gel Order:
-Ladder -J24679+B1002+K3 (A-E) -J24679+B1006+K3 (A-E)
The bands for 1B and 1D showed up so they were grown up accordingly.
July 27th
- Digests:
-