Team:TzuChiU Formosa/Meeting Minutes

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Revision as of 12:39, 4 August 2010

Our team has three sub-groups with different projects going on. The final project will be determined latest by August. Here are our meeting minutes starting from July.

Team 1: GoldenLi

Date: Jul-22, 2010

Last meeting, we try to use e-coli to secrete β-carotene, but we found that some biotech company had published this idea already.

So we decide to change our way:

• Use bacteria to deal the sewage problem
  
• Combine two igem biobrick, which are 2008 Chiba time manager and 2009UCSF Motile cellular robots.
  
• Use the system that the bacteria which can secrete the biofilm combined with our original idea that secretβ-carotene.
  
• use nano size silver ion to use at antibacterial
  
• creat a bacteria which could make there own Magnetic field
  

Date: Jul-19, 2010

• Team leader introduce subgroup project, the original idea came from Golden Rice production in Taiwan
• Plan to produce mass volume of beta-carotine in E-Coli to solve the problem of malnutrition in under-develope countries
• Current problem: B-carotine releasing mechanism
• Advisor: [http://www.humangenetics.tcu.edu.tw/new_faculty/wps/wps_basic.html Professor Woon Peng-Yeong, Institute of Molecular Biology and Human Genetics, Tzu Chi University]

Date: Jul-10, 2010

Our idea is using E.coli to synthesis β-carotene and secret it out of E.coli.
There are two main parts we have to do with this idea.

• Synthesisβ-carotene in E.coli. There’s a biobrick, BBa_K274210, which used to synthesize β-carotene is publish by 2009 Cambirdge team.
  
• The most difficult part of this idea is that we have to find the secretion mechanisms of E.coli, so this part divided into two major ways and we have to search for much more detail .
  

(1) Signal peptide: find a meet signal peptide for our idea. And find out where should it be cloned.
(2) E.coli: whether E.coli has the ability to exocytosis? Can it be used in secretion of β-carotene in E.coli?

Team 2: Poseidon - Save the World

Date: Jul-19, 2010

• Team leader introduces subgroup project, the project idea came from the recent massive oil leak in Gulf of Mexico
• Project introduction could be found here
• Plan to write to professor in German and Tianjin to obtain cyanobacteria and vectors for the project

Date: Jul-06, 2010

• Carry out experiment program:

 * Introduction: base on the PPT of first seminar, and do some correction.
 * Methods & materials:
   * Oil degradation genes: find out the institute to give us these genes.
   * Method of culturing cyanobacteria.
   * Method of transformation of cyanobacteria.
   * Detection device 1: detect the genes are successfully transform into cyanobacteria.
   * Detection device 2: detect if the oil-dgraded enzymes are workable.
 * Find an instructor:
   * Dr. Chen (department of lifescience)
   * Dr. Chang (department of laboratory medicine & biotechnology)

Date: Jun-18, 2010

• Rehearsal for the first idea seminar.
• First idea seminar.

Date: Jun-17, 2010

• Correct our idea model.
• List problems which need to be solve:

 * Which gene do we want to transform into cyanobacteria? (look up these genes’ name, size, and

function etc.)

 * Which strain of cyanobacteria we want to express oil-degraded-enzyme genes?
 * Look up the alkane transporter of A. borkumensis.
 * Look up the oil degradation enzyme genes.
 * Keep correcting the idea model.

Team 3: Saycar sata'os

Date: Jul-19, 2010

• Team leader introduce subgroup project, the project idea from the aromatic which put in bathroom.
• Plan to produce fragrance in E-Coli.
• Current problem: haven't find the sequence and the pathway.
• We have sent several letter to[http://bb.ncku.edu.tw/people/bio.php?PID=13 Professor Hong-Hwa Chen, Institute of Tropical Plant Sciences, NCKU]
• Advisor: [http://www.humangenetics.tcu.edu.tw/new_faculty/wps/wps_basic.html Professor Woon Peng-Yeong, Institute of Molecular Biology and Human Genetics, Tzu Chi University]