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Team:TU Delft/11 June 2010 content
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==Characterization of Anderson RBS sequences== | ==Characterization of Anderson RBS sequences== | ||
Next to that we also started digesting the first parts we will be characterizing. | Next to that we also started digesting the first parts we will be characterizing. | ||
| + | |||
| + | ==Strain Characterization== | ||
| + | By Hugo | ||
| + | |||
| + | I prepared the cell bank according to the following procedure: | ||
| + | |||
| + | * Spin 5 ml of the overnight culture | ||
| + | * Add 500 ul of LB medium | ||
| + | * Add 500 ul of 80% glycerol | ||
| + | * Store the tube at -80ºC | ||
| + | |||
| + | Now, we have a cell bank ready to use!!! | ||
=Spring Workshop= | =Spring Workshop= | ||
Revision as of 16:09, 25 August 2010
Contents |
Lab work
BioBrick stocks
Today we performed a Qiagen Mini-prep plasmid isolation of the vectors we grew overnight. We saved 3 mL of the bacterial cells to make -80 °C stocks.
The following plasmid concentrations were obtained:
| BioBrick | Concentration (ng/μL) |
| pSB3T5 | 266.0 |
| pSB1T3 | 414.0 |
| pSB1C3 | 284.7 |
| pSB3C5 | 329.3 |
| pSB1K3 | 270.1 |
| pSB1A3 | 271.9 |
| I13401 | 232.1 |
Characterization of Anderson RBS sequences
Next to that we also started digesting the first parts we will be characterizing.
Strain Characterization
By Hugo
I prepared the cell bank according to the following procedure:
- Spin 5 ml of the overnight culture
- Add 500 ul of LB medium
- Add 500 ul of 80% glycerol
- Store the tube at -80ºC
Now, we have a cell bank ready to use!!!
Spring Workshop
Almost the whole team left for the iGEM spring workshop in Évry, Paris. The drive for some of us was a bit laborious (traffic jams at the middle of the night/closed roads etc.), and others actually watched the World Cup soccer match... But in the end we all arrived at the hotel in Évry little before 2 AM, Saturday morning, and got some well deserved sleep.
