Team:UNIPV-Pavia/Calendar/August/settimana1

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both in vector <partinfo>pSB1A2</partinfo>.
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Transformation (1ul) of the following parts (resuspended/miniprepped):
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Transformation (1ul) of the following parts (resuspended/already miniprepped):
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Revision as of 15:35, 2 August 2010
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AUGUST: WEEK 1


August, 2nd

Miniprep and quantification at Nanodrop of:

  • I20-1: 98,2 ng/ul
  • I20-2: 63,6 ng/ul
  • I20-3: 41,5 ng/ul
  • I21-1: 45 ng/ul
  • I21-2: 45 ng/ul
  • I21-3: 54 ng/ul

These samples were prepared and sent (400ng) to BMR Genomics for sequencing.

The following parts were resuspended from iGEM 2010 Distribution Kit:

  • <partinfo>BBa_R0062</partinfo> (pLux; Plate 1, Well 6O)
  • <partinfo>BBa_K081008</partinfo> (RBS + lasI; Plate 2, Well 10N)

both in vector <partinfo>pSB1A2</partinfo>.

Transformation (1ul) of the following parts (resuspended/already miniprepped):

Part Strain
pAH123 MC1061
MG1655
<partinfo>BBa_J72008</partinfo> MC1061
MG1655
<partinfo>BBa_R0062</partinfo> DH5-alpha
<partinfo>BBa_K081008</partinfo> DH5-alpha

Transformed cells were plated on proper LB+Amp agar plate:

Part Plate resistance
pAH123 Amp 50 ug/ml
<partinfo>BBa_J72008</partinfo>
<partinfo>BBa_R0062</partinfo> Amp 100 ug/ml
<partinfo>BBa_K081008</partinfo>

Plates were let grow ON at 37°C.

August, 3rd

Check for plates grown ON:

August, 4th

August, 5th

August, 6th

August, 7th

August, 8th

Calendar

August

week 1

week 2

week 3

week 4

week 5

Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana1"