Team:UNIPV-Pavia/Calendar/August/settimana1

From 2010.igem.org

(Difference between revisions)
m (→August, 2nd)
(→August, 2nd)
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Transformation (1ul) of the following parts (resuspended/miniprepped):
Transformation (1ul) of the following parts (resuspended/miniprepped):
-
{|border='1' align='center'
+
{|border="1" align="center"
|-
|-
|'''Part''' || '''Strain'''
|'''Part''' || '''Strain'''
|-
|-
-
|pAH123 || MC1061
+
|rowspan="2"| pAH123 || MC1061
|-
|-
-
|pAH123 || MG1655
+
|MG1655
|-
|-
-
|<partinfo>BBa_J72008</partinfo> || MC1061
+
|rowspan="2"| <partinfo>BBa_J72008</partinfo> || MC1061
|-
|-
-
|<partinfo>BBa_J72008</partinfo> || MG1655
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|MG1655
|-
|-
|<partinfo>BBa_R0062</partinfo> || DH5-alpha
|<partinfo>BBa_R0062</partinfo> || DH5-alpha
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Transformed cells were plated on proper LB+Amp agar plate:
Transformed cells were plated on proper LB+Amp agar plate:
-
{|border='1' align='center'
+
{|border="1" align="center"
|-
|-
|'''Part''' || '''Plate resistance'''
|'''Part''' || '''Plate resistance'''
|-
|-
-
|pAH123 || Amp 50 ug/ml
+
|pAH123 ||rowspan="2"|  Amp 50 ug/ml
|-
|-
-
|pAH123 || Amp 50 ug/ml
+
|<partinfo>BBa_J72008</partinfo>
|-
|-
-
|<partinfo>BBa_J72008</partinfo> || Amp 50 ug/ml
+
|<partinfo>BBa_R0062</partinfo> || rowspan="2"| Amp 100 ug/ml
|-
|-
-
|<partinfo>BBa_J72008</partinfo> || Amp 50 ug/ml
+
|<partinfo>BBa_K081008</partinfo>
-
|-
+
-
|<partinfo>BBa_R0062</partinfo> || Amp 100 ug/ml
+
-
|-
+
-
|<partinfo>BBa_K081008</partinfo> || Amp 100 ug/ml
+
|-
|-
|}
|}

Revision as of 15:31, 2 August 2010
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AUGUST: WEEK 1


August, 2nd

Miniprep and quantification at Nanodrop of:

  • I20-1: 98,2 ng/ul
  • I20-2: 63,6 ng/ul
  • I20-3: 41,5 ng/ul
  • I21-1: 45 ng/ul
  • I21-2: 45 ng/ul
  • I21-3: 54 ng/ul

These samples were prepared and sent (400ng) to BMR Genomics for sequencing.

From iGEM 2010 Distribution Kit were resuspended the following parts:

  • <partinfo>BBa_R0062</partinfo> (pLux; Plate 1, Well 6O)
  • <partinfo>BBa_K081008</partinfo> (RBS + lasI; Plate 2, Well 10N)

both in vector <partinfo>pSB1A2</partinfo>.

Transformation (1ul) of the following parts (resuspended/miniprepped):

Part Strain
pAH123 MC1061
MG1655
<partinfo>BBa_J72008</partinfo> MC1061
MG1655
<partinfo>BBa_R0062</partinfo> DH5-alpha
<partinfo>BBa_K081008</partinfo> DH5-alpha

Transformed cells were plated on proper LB+Amp agar plate:

Part Plate resistance
pAH123 Amp 50 ug/ml
<partinfo>BBa_J72008</partinfo>
<partinfo>BBa_R0062</partinfo> Amp 100 ug/ml
<partinfo>BBa_K081008</partinfo>

August, 3rd

August, 4th

August, 5th

August, 6th

August, 7th

August, 8th

Calendar

August

week 1

week 2

week 3

week 4

week 5

Retrieved from "http://2010.igem.org/Team:UNIPV-Pavia/Calendar/August/settimana1"