Team:Cambridge/Quiescence/Sequences

From 2010.igem.org

(Difference between revisions)

Revision as of 18:35, 2 August 2010

Sequences

Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95:

AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUcuuggagcugaugagccguucgcggcgaaaacu aAGCUUAUUGCUugguuacuccaagAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCA UUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCGGUGCCUACAAU

This sequence does not include an aptamer.

Including aptamer

AgUcUgGuCuuCGCUuuuuCGCGUUCAacgCGAUCACccgAGCAGACAGGUucuAAUGGCAACA uuggACCCGucuAACUGgguCGAauuCGCCUggcgacccugaugaguuugaauaccagccgaaa ggcccuuggcagccaagcgaaacggugaaagccguagguugcccAGGCGCGAUCGCGGCAGUUU UUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUGAACGCGUUUUAGCG GUGCCUACAAU

Rcd sequences

Rcd 70

AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG

Rcd 95

AGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG AACGCGUUUUAGCGGUGCCUACAAU

Constructs

Rcd70_aptazyme_theo+_30_3 gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUU GAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG

Rcd70_aptazyme_theo+_28_9 CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCC UGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGUUGAGGCGC GAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG

Rcd70_aptazyme_theo+_30_8 CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCU GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG

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 Sequence for: Edited reverse Rcd complemement + ribozyme + Rcd95:
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 Rcd70_aptazyme_theo+_30_3
 gAGCGCcugCACGGCAGCgcuCAGGUAAAAccGGCAACuugaCAgguccAAggcaGCCuuuAUaaugcCU
-CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUUGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
+CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUA|GGUU
+GAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
 Rcd70_aptazyme_theo+_28_9
-CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU
+CAGCGCGAUCACGuCAGCAcuguGGUAAuuccGGCAAgugguuACCCGAuaucacGCCuauAUCGCaggc|CGACCC
-UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
+UGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGUUGAGGCGC
+GAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
 Rcd70_aptazyme_theo+_30_8
-CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCUGAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU
+CAGCGCuAUCACGGugGCAcugucGUAAuuccGGCAAgugguCACCCGAuaucaUGCCuaucUCGCaggc|CGACCCU
+GAUGAGUUUGAAUACCAGCCGAAAGGCCCUUGGCAGCCAAGCGAAACGGUGAAAGCCGUAGGU
 UGAGGCGCGAUCGCGGCAGUUUUUCGGGUGGUUUGUUGCCAUUUUUACCUGUCUGCUGCCGUGAUCGCGCUG
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