"
Team:TU Delft/21 July 2010 content
From 2010.igem.org
(Difference between revisions)
| Line 30: | Line 30: | ||
|220 | |220 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
| - | |<font color=limegreen> | + | |<font color=limegreen>✓</font> |
| | | | ||
|- | |- | ||
| Line 37: | Line 37: | ||
|300 | |300 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
| - | |<font color=red> | + | |<font color=red>✗</font> |
| | | | ||
|- | |- | ||
| Line 44: | Line 44: | ||
|300 | |300 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
| - | |<font color=red> | + | |<font color=red>✗</font> |
| | | | ||
|- | |- | ||
| Line 51: | Line 51: | ||
|300 | |300 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
| - | |<font color=red> | + | |<font color=red>✗</font> |
| | | | ||
|- | |- | ||
| Line 58: | Line 58: | ||
|300 | |300 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
| - | |<font color=red> | + | |<font color=red>✗</font> |
| | | | ||
|- | |- | ||
| Line 65: | Line 65: | ||
|300 | |300 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
| - | |<font color=red> | + | |<font color=red>✗</font> |
| | | | ||
|- | |- | ||
| Line 72: | Line 72: | ||
|300 | |300 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
| - | |<font color=red> | + | |<font color=red>✗</font> |
| | | | ||
|- | |- | ||
| Line 79: | Line 79: | ||
|300 | |300 | ||
|G00100 + G00101 | |G00100 + G00101 | ||
| - | |<font color=red> | + | |<font color=red>✗</font> |
| | | | ||
|} | |} | ||
Unfortunately all other lanes were empty or the same as the negative control. So from this gel we conclude that the ligation has failed. | Unfortunately all other lanes were empty or the same as the negative control. So from this gel we conclude that the ligation has failed. | ||
Revision as of 18:56, 2 August 2010
Contents |
Lab work
Ordered DNA
The transformations of 19 July containing the different ligations gave colonies (~5 per plate). We picked 5 colonies per plate and performed a colony PCR.
Alkane degradation
Unfortunately there were no transformants on yesterday's plates. This is most likely due to the fact that the ligation didn't work with the small pieces of DNA of the RBSs. Next plan is to cut open the RBS plasmid without removing the RBS (with SpeI and PstI) and insert the gene in this plasmid. We will try this tomorrow.
Emulsifier
Lane Description
| # | Description | Expected lenght (bp) | Primer | Status | Remarks |
| M1 | EZ Ladder | n/a | n/a | n/a | |
| 1 | B0032 (control) | 220 | G00100 + G00101 | ✓ | |
| 2 | Transformant #1 of ligation mix R0011-B0032 | 300 | G00100 + G00101 | ✗ | |
| 3 | Transformant #2 of ligation mix R0011-B0032 | 300 | G00100 + G00101 | ✗ | |
| 4 | Transformant #3 of ligation mix R0011-B0032 | 300 | G00100 + G00101 | ✗ | |
| 5 | Transformant #4 of ligation mix R0011-B0032 | 300 | G00100 + G00101 | ✗ | |
| 6 | Transformant #5 of ligation mix R0011-B0032 | 300 | G00100 + G00101 | ✗ | |
| 7 | Transformant #6 of ligation mix R0011-B0032 | 300 | G00100 + G00101 | ✗ | |
| 8 | Transformant #7 of ligation mix R0011-B0032 | 300 | G00100 + G00101 | ✗ |
Unfortunately all other lanes were empty or the same as the negative control. So from this gel we conclude that the ligation has failed.
