HKU-Hong Kong/13 July 2010

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=13 July 2010:Con't BBa_k112808 isolation=
=13 July 2010:Con't BBa_k112808 isolation=
Different combinations of enzymes were used for the isolation.
Different combinations of enzymes were used for the isolation.
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The results of single enzyme digestion were the same for double enzyme digestion, suggesting that one of the site might be absent.
The results of single enzyme digestion were the same for double enzyme digestion, suggesting that one of the site might be absent.
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Revision as of 18:01, 27 October 2010

13 July 2010:Con't BBa_k112808 isolation

Different combinations of enzymes were used for the isolation.

Result

results of digestions

Controls were run in parallel to the plasmid after various treatments. An upward shift indicated the presence of a functional cutting site to turn the faster-moving circular plasmid to slower-moving linear DNA fragments. The results of single enzyme digestion were the same for double enzyme digestion, suggesting that one of the site might be absent.

 

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