HKU-Hong Kong/13 July 2010
From 2010.igem.org
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[[Image:13jul.jpg|500px|results of digestions]] | [[Image:13jul.jpg|500px|results of digestions]] | ||
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Controls were run in parallel to the plasmid after various treatments. | Controls were run in parallel to the plasmid after various treatments. | ||
An upward shift indicated the presence of a functional cutting site to turn the faster-moving circular plasmid to slower-moving linear DNA fragments. | An upward shift indicated the presence of a functional cutting site to turn the faster-moving circular plasmid to slower-moving linear DNA fragments. |
Revision as of 08:17, 31 July 2010
13 July 2010:Con't BBa_k112808 isolation
Different combinations of enzymes were used for the isolation.
Result
Controls were run in parallel to the plasmid after various treatments. An upward shift indicated the presence of a functional cutting site to turn the faster-moving circular plasmid to slower-moving linear DNA fragments. The results of single enzyme digestion were the same for double enzyme digestion, suggesting that one of the site might be absent.