Team:Michigan/Protocols

From 2010.igem.org

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(Protocols)
(Quorum Sensing)
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[[Media:Quorum+Sensing+Tables-1-.pdf|Logic Table]]
[[Media:Quorum+Sensing+Tables-1-.pdf|Logic Table]]
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[[Media:QS_Procedures-1-.pdf|Experimental Protocols]]
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[[Media:QS_Procedures-1-.pdf|Lsr Circuit Test Protocols]]

Revision as of 17:51, 30 July 2010


Michigan Header




General Outline for Biobrick Manipulations

Igem order of procedures.jpg

Protocols

Using Lab Equipment

Obtaining Deionized Water in the ERB

ERB Spectrophotometer

Enigeering Research Building Autoclave

Epifluorescence Microscope Usage - H.H. Dow

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

Culturing CGSC Strains

OD600 Cell Dilution Protocol

DNA Manipulation

Transformation-heat shock

This is the procedure we can perform in the ERB

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Heat shock

Transformation-electroporation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

  • Ginko Bioworks Protocol
  • Jeremy Minty Protocol

Group-Specific Protocols

Quorum Sensing

Logic Table

Lsr Circuit Test Protocols