Team:ETHZ Basel/Biology

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Revision as of 11:46, 28 July 2010

As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).

Currently we are working on putting all the BioBricks into a storage vector. The image

alt text

shows all the constructs we plan to clone.

Revision as of 11:45, 28 July 2010 (view source) Zwickyk (Talk \| contribs) ← Older edit		Revision as of 11:46, 28 July 2010 (view source) Zwickyk (Talk \| contribs) Newer edit →
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	As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).		As we plan to generate several fusion proteins with different linkers, we decided to use the cloning strategy BBF RFC 28: A method for combinatorial multi-part assembly based on the Type IIs restriction enzyme AarI (http://dspace.mit.edu/handle/1721.1/46721).

-	Currently we are working on putting all the BioBricks into a storage vector. The image [[Image:Constructs.jpg\|200px\|thumb\|right\|alt text]] shows all the constructs we plan to clone.	+	<br>Currently we are working on putting all the BioBricks into a storage vector. The image [[Image:Constructs.jpg\|200px\|thumb\|right\|alt text]] shows all the constructs we plan to clone.