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Team:Stockholm/26 July 2010
From 2010.igem.org
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(New page: == Mimmi == === LMWP C and N === Plasmid prep and gycerol stock The cells were cultured to 0,6 OD, 1,6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid ''...) |
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=== LMWP C and N === | === LMWP C and N === | ||
| - | Plasmid prep and | + | Plasmid prep and glycerol stock |
| - | The cells were cultured to 0 | + | The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid |
Revision as of 09:05, 29 July 2010
Mimmi
LMWP C and N
Plasmid prep and glycerol stock
The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid
DNA concentrations
| Sample | DNA (ng/μl) |
| LMWP N | 60 |
| LMWP C | 80 |
over-expression in pEX - remaking the comassie-gel
MIX:
- 4ml LB
- 40µl glycose (1%)
- 8µl Amp (0,5%)
Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON
