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Team:Davidson-MissouriW/CreLox
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<p> The Cre-lox tool is a site-specific recombination system that is widely used in biological research to manipulate DNA. It was discovered in the early 90's through characterization of coliphage P1 recombination system. The Cre recombinase enzyme, a 38kDa protein, catalyzes the recombination of DNA between two lox sites. These lox sites, each 34 bp long, consist of two inverted repeat arms flanking a spacer region of 8bp that is unique to the lox site.</p> | <p> The Cre-lox tool is a site-specific recombination system that is widely used in biological research to manipulate DNA. It was discovered in the early 90's through characterization of coliphage P1 recombination system. The Cre recombinase enzyme, a 38kDa protein, catalyzes the recombination of DNA between two lox sites. These lox sites, each 34 bp long, consist of two inverted repeat arms flanking a spacer region of 8bp that is unique to the lox site.</p> | ||
| - | <img border="black" src="https://static.igem.org/mediawiki/2010/0/07/Davidson-MissouriWcre-lox.jpeg"><img src="https://static.igem.org/mediawiki/2010/d/d5/Davidson-MissouriWinversion.jpeg"> | + | <table> |
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| + | <img border="black" src="https://static.igem.org/mediawiki/2010/0/07/Davidson-MissouriWcre-lox.jpeg"> | ||
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| + | <img src="https://static.igem.org/mediawiki/2010/d/d5/Davidson-MissouriWinversion.jpeg"> | ||
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Revision as of 19:43, 27 July 2010
iGEM Davidson – MWSU 2010: Characterizing Cre/lox Recombination Method
The Cre-lox tool is a site-specific recombination system that is widely used in biological research to manipulate DNA. It was discovered in the early 90's through characterization of coliphage P1 recombination system. The Cre recombinase enzyme, a 38kDa protein, catalyzes the recombination of DNA between two lox sites. These lox sites, each 34 bp long, consist of two inverted repeat arms flanking a spacer region of 8bp that is unique to the lox site.
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