"

From 2010.igem.org

Revision as of 16:29, 27 July 2010 (view source) Annlesn (Talk | contribs) ← Older edit		Revision as of 00:22, 2 August 2010 (view source) Annlesn (Talk | contribs) Newer edit →
Line 27:		Line 27:
	'''0.1 M CaCl2 solution'''		'''0.1 M CaCl2 solution'''
	*0.555 g of CaCl2		*0.555 g of CaCl2
		+	*50 mL of DI water
		+	''filter sterilize''
		+
		+	'''1.0 M CaCl2 solution'''
		+	*5.55 g of CaCl2
	*50 mL of DI water		*50 mL of DI water
	''filter sterilize''		''filter sterilize''

---

Revision as of 00:22, 2 August 2010

Media Recipes

LB media

• 10 g LB broth
• 500 mL DI water

autoclave to sterilize

LB media with agar

• 10 g LB broth
• 7.5 g agar
• 500 mL DI water

autoclave to sterilize

add antibiotics after autoclaving when warm to touch (about 50°C)

pour plates when warm to the touch (about 50°C)

0.1 M CaCl2 solution

• 0.555 g of CaCl2
• 50 mL of DI water

filter sterilize

1.0 M CaCl2 solution

• 5.55 g of CaCl2
• 50 mL of DI water

filter sterilize

Ampicillin Antibiotic Stock Solution (100 mg/mL)

• 1 g of ampicillin
• 5 mL of DI water
• 5 mL of ethanol

filter sterilize and make 1 mL aliquotes to store in the -20°C freezer

Kanamycin Antibiotic Stock Solution (50 mg/mL)

• 250 mg of kanamycin
• 5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

• 250 mg of tetracycline
• 2.5 mL of DI water
• 2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20°C freezer

IPTG stock solution (1 M)

• 0.25 g of IPTG
• 1049 uL of DI water

filter sterilize with .22 um filter