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Team:Harvard/vectors
From 2010.igem.org
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<div id="abstract"> | <div id="abstract"> | ||
<h1>abstract</h1> | <h1>abstract</h1> | ||
| - | <p>Our goal is the creation of an "iGarden" with the | + | <p>Our goal is the creation of an "iGarden" with the BioBrick standard. For such a concept to work, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli into the plant arabidopsis.</p> |
| - | <p> | + | <p>The process began with the creation of a set of <a href="https://2010.igem.org/Team:Harvard/vectors/vectors">plant vectors</a> compatible with the BioBrick standard. To express our constucts - such as <a href="https://2010.igem.org/Team:Harvard/flavor">flavor</a> elements, <a href="https://2010.igem.org/Team:Harvard/allergy">allergen</a> knockdown, <a href="https://2010.igem.org/Team:Harvard/color">pigment</a> accumulation, or <a href="https://2010.igem.org/Team:Harvard/fences">fence</a> mechanisms, we cloned the constucts into the agrobacterium vectors with E. coli as the chassis, then transformed into agrobacteria via electroporation, then innoculated our plants with the contruct containing agrobacteria. </p> |
<div> | <div> | ||
<img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"> | <img src="https://static.igem.org/mediawiki/2010/0/03/Harvard2010roadmap.png" width="600px"> | ||
Revision as of 17:16, 14 October 2010
abstract lab notebook agrobacteria and plant engineering meet the vectors plant transformation parts & primers references
abstract
Our goal is the creation of an "iGarden" with the BioBrick standard. For such a concept to work, we used agrobacterium-mediated transformation to move our constructs from the bacterium e. coli into the plant arabidopsis.
The process began with the creation of a set of plant vectors compatible with the BioBrick standard. To express our constucts - such as flavor elements, allergen knockdown, pigment accumulation, or fence mechanisms, we cloned the constucts into the agrobacterium vectors with E. coli as the chassis, then transformed into agrobacteria via electroporation, then innoculated our plants with the contruct containing agrobacteria.
