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Team:Newcastle/26 July 2010
From 2010.igem.org
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# For the full protocol, please refer to Colony PCR in Protocol List. | # For the full protocol, please refer to Colony PCR in Protocol List. | ||
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# Melting temperature, Tm used for Anneal step is 59°C. | # Melting temperature, Tm used for Anneal step is 59°C. | ||
Revision as of 14:08, 26 July 2010
Contents |
Colony PCR of Genomic DNA
Aim:
To determine whether the genes have been inserted into the plasmid of B. Subtilis 168.
Materials:
- Pipette
- Microfuge
- Microtubes
- Distilled H2O
- Nucleotide DNTP
- 5x GoTaq buffer
- Template DNA (B. Subtilis ATCC 6633, 1:1 and 1:2)
- Forward and reverse primers
Protocol:
- For the full protocol, please refer to Colony PCR in Protocol List.
Conditions in ThermoCycler:
- Melting temperature, Tm used for Anneal step is 59°C.
