"
Team:Newcastle/26 July 2010
From 2010.igem.org
(Difference between revisions)
(→Materials:) |
(→Protocol:) |
||
| Line 18: | Line 18: | ||
====Protocol:==== | ====Protocol:==== | ||
| - | # | + | # For the full protocol, please refer to Colony PCR in Protocol List. |
# Melting temperature, Tm used for Anneal step is 59°C. | # Melting temperature, Tm used for Anneal step is 59°C. | ||
| - | |||
Revision as of 14:06, 26 July 2010
Contents |
Colony PCR of Genomic DNA
Aim:
To determine whether the genes have been inserted into the plasmid of B. Subtilis 168.
Materials:
- Pipette
- Microfuge
- Microtubes
- Distilled H2O
- Nucleotide DNTP
- 5x GoTaq buffer
- Template DNA (B. Subtilis ATCC 6633, 1:1 and 1:2)
- Forward and reverse primers
Protocol:
- For the full protocol, please refer to Colony PCR in Protocol List.
- Melting temperature, Tm used for Anneal step is 59°C.
