Team:Newcastle/26 July 2010

From 2010.igem.org

(Difference between revisions)
(New page: ===Colony PCR of Genomic DNA=== ====Materials:==== # Materials added according to Colony PCR on Protocol list. # Melting temperature, Tm used for Anneal step is 59°C.)
Line 1: Line 1:
===Colony PCR of Genomic DNA===
===Colony PCR of Genomic DNA===
 +
 +
====Aim:====
 +
 +
To determine whether the genes have been inserted into the plasmid of ''B. Subtilis 168''.
====Materials:====
====Materials:====
 +
* Pipette
 +
* Microfuge
 +
* Microtubes
 +
* Distilled H2O
 +
* Nucleotide DNTP
 +
* 5x GoTaq buffer
 +
* Template DNA (B. Subtilis ATCC 6633, 1:1 and 1:2)
 +
* Forward and reverse primers
# Materials added according to Colony PCR on Protocol list.
# Materials added according to Colony PCR on Protocol list.
# Melting temperature, Tm used for Anneal step is 59°C.
# Melting temperature, Tm used for Anneal step is 59°C.
 +
#

Revision as of 14:02, 26 July 2010

Colony PCR of Genomic DNA

Aim:

To determine whether the genes have been inserted into the plasmid of B. Subtilis 168.

Materials:

  • Pipette
  • Microfuge
  • Microtubes
  • Distilled H2O
  • Nucleotide DNTP
  • 5x GoTaq buffer
  • Template DNA (B. Subtilis ATCC 6633, 1:1 and 1:2)
  • Forward and reverse primers
  1. Materials added according to Colony PCR on Protocol list.
  2. Melting temperature, Tm used for Anneal step is 59°C.
Retrieved from "http://2010.igem.org/Team:Newcastle/26_July_2010"