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Team:Michigan/Josh's Notebook
From 2010.igem.org
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lane 9 - OmpA (K103006) | lane 9 - OmpA (K103006) | ||
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• aliquot INP samples and primers for sequencing | • aliquot INP samples and primers for sequencing | ||
Revision as of 01:43, 26 July 2010
7/10/2010
13:00
• digest I719015 (T7 GFPmut3b) and I719005(T7 Promoter) with EcoR1 and Pst1.
7/13/2010
9:00
• inoculate competent cell culture
• harvest I719015, K145001, K117008, K117002, K103006 from registry
7/15/2010
4:30
• streak plate INP NC sample
7/18/2010
5:00
• miniprep INP NC sample
7/21/2010
6:00
• digest INP NC with EcoRI and SpeI
7/23/2010
9:00
• gel electrophoresis of all biobricks
lane 1 - ladder
lane 2 - INP NC sample 1 (K265008)
lane 3 - INP NC sample 2 (K265008)
lane 4 - T7 GFPmut3b (I719015)
lane 5 - T7 Promoter (I719005)
lane 6 - T7 RNA polymerase (K145001)
lane 7 - pLsrA-YFP (K117008)
lane 8 - LsrA promoter (K117002)
lane 9 - OmpA (K103006)
7/25/2010
5:00
• aliquot INP samples and primers for sequencing
