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Team:EPF Lausanne/Labbook protocols
From 2010.igem.org
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| - | = | + | = Lab Protocols = |
| + | |||
| + | On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | ||
| + | |||
| + | |||
| + | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_adapt_pH.pdf Adapt pH] | ||
| + | *[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel] | ||
| + | *[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production] | ||
| + | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ] | ||
| + | *Competence Asaia (Nadine) | ||
| + | *[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | ||
| + | * Growing Asaia (Effie) | ||
| + | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation] | ||
| + | *[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop] | ||
| + | * Miniprep --> use Miniprep Kit | ||
| + | *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)] | ||
| + | *[https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR ] | ||
| + | * Purification --> use Purification Kit | ||
| + | *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells] | ||
| + | *[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging] | ||
| + | *Using the plate reader (Solange) | ||
| + | *[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion] | ||
| + | |||
| + | = Media Recipes = | ||
| + | *[https://static.igem.org/mediawiki/2010/archive/e/ed/20100715125103!Prot_LB.pdf LB medium] | ||
| + | *[https://static.igem.org/mediawiki/2010/5/52/Prot_LB_agar.pdf LB agar medium] | ||
| + | *[https://static.igem.org/mediawiki/2010/1/16/Prot_gly.pdf Gly medium] | ||
| + | *[https://static.igem.org/mediawiki/2010/0/0e/Prot_gly_agar.pdf Gly agar medium] | ||
| + | *[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium] | ||
| + | |||
| + | = Syntax = | ||
| + | convention if you want to upload a new protocol. | ||
| + | |||
| + | When uploading a file it please rename it in the following way: <i> EPFL_prot_yourProcedureName.yourExtension</i>. | ||
</div> | </div> | ||
Revision as of 22:04, 23 July 2010
Lab Protocols
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.
- Adapt pH
- Agarose Gel
- Agar plates production
- Autoclave
- Competence Asaia (Nadine)
- Electrophoresis
- Growing Asaia (Effie)
- Ligation
- Measuring DNA concentration using NanoDrop
- Miniprep --> use Miniprep Kit
- Optical Density (OD)
- PCR
- Purification --> use Purification Kit
- Transformation of competent cells
- UV Imaging
- Using the plate reader (Solange)
- Digestion
Media Recipes
Syntax
convention if you want to upload a new protocol.
When uploading a file it please rename it in the following way: EPFL_prot_yourProcedureName.yourExtension.
