NanoDrop Spectrophotometer

From 2010.igem.org

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Nanodrop can be used to measure the DNA, RNA and protein
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Measure the concentration and purity of extracted DNA using absorbance (using the automated nanodrop machine!)
Measure the concentration and purity of extracted DNA using absorbance (using the automated nanodrop machine!)
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#Log onto computer, open program ND 1000
#Log onto computer, open program ND 1000
#Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program
#Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program
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#Wipe, apply elution buffer (EB) or appropriate blank control (3 µl) to nib, blank the system, set to DNA-50 for DNA.
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#Wipe, apply elution buffer (EB) or appropriate blank control (3 µl) to nib, blank the system, set to DNA-50 for DNA.  
#Wipe, apply sample to nib, measure  
#Wipe, apply sample to nib, measure  
#Reblank about every 10 samples
#Reblank about every 10 samples
#Blank with water at end, wipe and log off
#Blank with water at end, wipe and log off
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# Log onto computer and select Nanodrop program from the desktop (ND 1000)
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# To clean Nanodrop wipe pedestal and top and add 3 µl of water to nib of pedestal on the spectrometer and press blank.
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# Wipe the water off, to initialise/equalizen the equipment add 3 μl of the elution buffer [EB] used in the sample and press blank. Set to DNA-50 for DNA.
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# Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
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# If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
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# After measurement, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.
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{|
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|[[Image:Newcastle_nanodrop_1.jpeg|thumb]]
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|[[Image:Newcastle_nanodrop_2.jpeg|thumb]]
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|[[Image:Newcastle_nanodrop_3.jpeg|thumb]]
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Revision as of 08:59, 22 July 2010

Nanodrop can be used to measure the DNA, RNA and protein

Measure the concentration and purity of extracted DNA using absorbance (using the automated nanodrop machine!)

The ideal concentration of DNA is 150 ng/ml!

Method:

  1. Log onto computer, open program ND 1000
  2. Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program
  3. Wipe, apply elution buffer (EB) or appropriate blank control (3 µl) to nib, blank the system, set to DNA-50 for DNA.
  4. Wipe, apply sample to nib, measure
  5. Reblank about every 10 samples
  6. Blank with water at end, wipe and log off



  1. Log onto computer and select Nanodrop program from the desktop (ND 1000)
  2. To clean Nanodrop wipe pedestal and top and add 3 µl of water to nib of pedestal on the spectrometer and press blank.
  1. Wipe the water off, to initialise/equalizen the equipment add 3 μl of the elution buffer [EB] used in the sample and press blank. Set to DNA-50 for DNA.
  1. Wipe to remove buffer and apply 3 μl of sample to nib. Press measure.
  2. If dealing with multiple samples, clean the equipment with water at regular intervals (about every 10 samples)
  3. After measurement, clean the equipment with 3 μl of water on the spectrometer and press blank. Wipe and log off.


Newcastle nanodrop 1.jpeg
Newcastle nanodrop 2.jpeg
Newcastle nanodrop 3.jpeg
Retrieved from "http://2010.igem.org/NanoDrop_Spectrophotometer"