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Revision as of 08:25, 22 July 2010 (view source) Yessa (Talk | contribs) ← Older edit		Revision as of 08:34, 22 July 2010 (view source) Yessa (Talk | contribs) Newer edit →
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	Method:		Method:
-	Log onto computer, open program ND 1000	+	#Log onto computer, open program ND 1000
-	Wipe pedestal and top, apply 3	+	#Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program
		+	#Wipe, apply EBor appropriate blank control (3 µl) to nib, blank the system, set to DNA-50 for DNA.
		+	#Wipe, apply sample to nib, measure
		+	#reblank about every 10 samples
		+	#blank with water at end, wipe and log off
		+
		+	Ideal concentration is 150 ng/ml

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Revision as of 08:34, 22 July 2010

Measure the concentration and purity of extracted DNA using absorbance (automated nanodrop machine)

Method:

1. Log onto computer, open program ND 1000
2. Wipe pedestal and top. Apply 3 µl of water to nib of pedestal and initiliase program
3. Wipe, apply EBor appropriate blank control (3 µl) to nib, blank the system, set to DNA-50 for DNA.
4. Wipe, apply sample to nib, measure
5. reblank about every 10 samples
6. blank with water at end, wipe and log off

Ideal concentration is 150 ng/ml