Team:Stanford/Notebook/Summaries
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==Timeline for Team Analog== | ==Timeline for Team Analog== | ||
- | *[Team:Stanford/Notebook/Lab_Work/Week_1 | Week 1: 6-28-2010] | + | *[[Team:Stanford/Notebook/Lab_Work/Week_1 | Week 1: 6-28-2010]] |
**[x] Get parts and competent cells. | **[x] Get parts and competent cells. | ||
**[x] Culture parts. | **[x] Culture parts. |
Revision as of 17:51, 20 July 2010
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Spring: Brainstorming | Spring Meetings
Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries
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Timeline for Team Analog
- Week 1: 6-28-2010
- [x] Get parts and competent cells.
- [x] Culture parts.
- [x] Miniprep parts/make stocks.
- [_] Characterize parts (promoters).
- Week 2: 7-05-2010
- [x] Restriction digest parts.
- [x] PCR superfolded GFP reporter.
- [x] Gel extract parts.
- [_] Order genes for kinases, phosphatases, and transcription factors.
- [_] Characterize parts (promoters).
- Week 3: 7-12-2010
- [x] Ligate parts.
- [x] Order primers for small parts and promoters.
- [x] Prepare MDV presentation and get money (hopefully).
- [x] Order genes for kinases, phosphatases, and transcription factors.
- [_] Streak BW27783 and culture to lose its current plasmid.
- [_] Characterize parts (promoters).
- Week 4: 7-19-2010
- [_] Receive primers and build promoters.
- [_] Streak BW27783 and culture to lose its current plasmid.
- [_] Transform ligations and conduct sequencing.
- [_] Characterize parts (promoters).
- [_] Try chromosomal integration proposed by Francois (for TF proomoter and reporter maybe?).
- [_] Order more primers (degradation tags, truncated proteins, etc.).
- Week 5: 7-26-2010
- [_] Receive protein parts and finish with assembly.
- [_] Characterize protein parts (conduct kinetic assays, in vivo and in vitro).
- [_] Do other promoter stuff (mutagenesis for better linearity, substrate specificity, attempt stoplight problem?)
- Week 6: 8-02-2010
- [_] Improve design.
- Week 7: 8-09-2010
- [_] Win iGEM. Already.
- Week 8: 8-16-2010
- Week 9: 8-23-2010
- Week 10: 7-30-2010
- NOTE: Wednesday (8-1-2010). Housing contracts expire; move out by noon.