Team:Stanford/Notebook/Lab Work/Week 2

From 2010.igem.org

(Difference between revisions)
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==7/1 Thursday==
==7/1 Thursday==
-
Laura: did 8 minipreps from Chris'/Alex's cultures
+
Laura:  
 +
*did 8 minipreps from Chris'/Alex's cultures
used Qiagen spin kit, with the following variations:
used Qiagen spin kit, with the following variations:
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#eluted with H2O
#eluted with H2O
-
*Concentrations from Nanodrop (taken by Chris B.)
+
**Concentrations from Nanodrop (taken by Chris B.)
-
**psb1A2: 58 ng/uL
+
***psb1A2: 58 ng/uL
-
**psb1A3: 53 ng/uL
+
***psb1A3: 53 ng/uL
-
**psb2K3: 40 ng/uL
+
***psb2K3: 40 ng/uL
-
**psb4A5: 63.4 ng/uL
+
***psb4A5: 63.4 ng/uL
-
**E0240: 65 ng/uL
+
***E0240: 65 ng/uL
-
**T9002: 104 ng/uL
+
***T9002: 104 ng/uL
-
**F2620: 57.5ng/uL
+
***F2620: 57.5ng/uL
-
helped Karina, Francisco with the transformations of parts
+
*helped Karina, Francisco with the transformations of parts
</div>
</div>

Revision as of 23:58, 19 July 2010

Quad center.jpg

Spring: Brainstorming | Spring Meetings

Summer: Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Summaries


Contents

7/1 Thursday

Laura:

  • did 8 minipreps from Chris'/Alex's cultures

used Qiagen spin kit, with the following variations:

  1. skipped "recommended" wash step
  2. eluted with H2O
    • Concentrations from Nanodrop (taken by Chris B.)
      • psb1A2: 58 ng/uL
      • psb1A3: 53 ng/uL
      • psb2K3: 40 ng/uL
      • psb4A5: 63.4 ng/uL
      • E0240: 65 ng/uL
      • T9002: 104 ng/uL
      • F2620: 57.5ng/uL
  • helped Karina, Francisco with the transformations of parts