Team:Stanford/NotebookPage/13 July 2010

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(Difference between revisions)
(New page: '''Christopher's Lab Notebook for 7/13/10''' *Run Gels (0.8%) of Restriction Digests: 1M, 2M, 2J (X/P) F2620 (E/S) 3C5 (E/P) I0500 (E/S) '''Note: for PCR of I746908: after first ...)
 
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'''Note: for PCR of I746908: after first  gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation'''.
'''Note: for PCR of I746908: after first  gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation'''.
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==Laura's Lab notebook==
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*restreaked plates from 7/1/10 (with Karina)
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*for each plate, pick 8 colonies and streak, starting at the center of the "pie piece," and working towards the perimeter of the plate
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*plate ID's:
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Latest revision as of 21:27, 19 July 2010

Christopher's Lab Notebook for 7/13/10

  • Run Gels (0.8%) of Restriction Digests: 
 1M, 2M, 2J (X/P)
 F2620 (E/S)
 3C5 (E/P)
 I0500 (E/S)

Note: for PCR of I746908: after first gel of PCR product, gel extract, and then do a restriction digest. After restriction digest, do a reaction cleanup; pieces are ready for ligation.


Laura's Lab notebook

  • restreaked plates from 7/1/10 (with Karina)
  • for each plate, pick 8 colonies and streak, starting at the center of the "pie piece," and working towards the perimeter of the plate
  • plate ID's:
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