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Team:EPF Lausanne/Protocols
From 2010.igem.org
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On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | ||
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_adapt_pH.pdf Adapt pH] | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_adapt_pH.pdf Adapt pH] | ||
| - | *[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel | + | *[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel] |
| - | *[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production | + | *[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production] |
| - | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave | + | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ] |
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | *[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | ||
* Asaia Maxiprep (effie) | * Asaia Maxiprep (effie) | ||
* Asaia Miniprep (effie) | * Asaia Miniprep (effie) | ||
| - | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation | + | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation] |
*[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop] | *[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop] | ||
| - | *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf OD | + | *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)] |
| - | *[https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR | + | *[https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR ] |
* Purification --> purification Kit | * Purification --> purification Kit | ||
| - | *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells | + | *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells] |
* UV imaging (Wiktor) | * UV imaging (Wiktor) | ||
* Using the plate reader (Solange) | * Using the plate reader (Solange) | ||
Revision as of 13:31, 19 July 2010
Lab Protocols
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.
- Adapt pH
- Agarose Gel
- Agar plates production
- Autoclave
- Electrophoresis
- Asaia Maxiprep (effie)
- Asaia Miniprep (effie)
- Ligation
- Measuring DNA concentration using NanoDrop
- Optical Density (OD)
- PCR
- Purification --> purification Kit
- Transformation of competent cells
- UV imaging (Wiktor)
- Using the plate reader (Solange)
Media Recipes
Syntax: convention if you want to upload a new protocol
When uploading a file it please rename it in the following way: prot_yourProcedureName.yourExtension.
