Team:EPF Lausanne/Protocols

From 2010.igem.org

(Difference between revisions)
Line 3: Line 3:
== Lab Protocols ==
== Lab Protocols ==
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.  
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.  
 +
* adapt ph (Nadine)
*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel protocol]
*[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel protocol]
-
*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf OD protocol]
 
-
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave protocol]
 
*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production protocol]
*[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production protocol]
-
* adapt ph (Nadine)
+
*[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave protocol]
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
-
* UV imaging (Wiktor)
 
-
* [https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
 
-
* [https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR protocol ]
 
-
* Using the plate reader (Solange)
 
* Asaia Maxiprep (effie)
* Asaia Maxiprep (effie)
* Asaia Miniprep (effie)
* Asaia Miniprep (effie)
-
* [https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation protocol]
+
*[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation protocol]
-
* [https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells protocol ]
+
*[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
 +
*[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf OD protocol]
 +
*[https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR protocol ]
* purification --> purification Kit
* purification --> purification Kit
 +
*[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells protocol ]
 +
* UV imaging (Wiktor)
 +
* Using the plate reader (Solange)
Media Recipes
Media Recipes

Revision as of 12:50, 19 July 2010

home

Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Media Recipes


Syntax: convention if you want to upload a new protocol

When uploading a file it please rename it in the following way:  prot_yourProcedureName.yourExtension.