"
Team:Newcastle/15 July 2010
From 2010.igem.org
(Difference between revisions)
(→Summary) |
|||
| Line 2: | Line 2: | ||
Screening for lacI insert: | Screening for lacI insert: | ||
| - | + | #(Miniprep) | |
#Restriction digests | #Restriction digests | ||
#*Single digest with Pst1 | #*Single digest with Pst1 | ||
Revision as of 10:52, 19 July 2010
Summary
Screening for lacI insert:
- (Miniprep)
- Restriction digests
- Single digest with Pst1
- double digests with Pst1 and Xba1
- Gel electrophoresis
Set up liquid broth culture in LB
