Team:Alberta/Plates

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Revision as of 18:28, 16 July 2010

Notebook	Building Parts	Testing Parts	Assembly Method	Competent Cells	Plates	Kit Manual	Software

Plates

L.B.O.

24-06-10

Creation of "L.B.O.": a deodorant stick of LB agar

All of the following steps were performed in a safety cabinet under sterile conditions.

1) disassembled a Degree deodorant stick and soaked in ethanol

2) removed the raising platform and covered it with Parafilm

3) coated the insides of the tube with mineral oil

5) removed platform and poured LB agar into the base of the stick, waited until it solidified

6) the Parafilmed platform was put back into the stick and lowered maximally

7) LB agar was poured on top of the platform, until the tube was full, waited until it solidifed

8) The top of the LB agar was sliced off with an ethanol-sterilized knife

9) 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate

10) L.B.O. stick was incubated overnight at 37C

@@ Line 20: / Line 20: @@
 <p>
 All of the following steps were performed in a safety cabinet under sterile conditions.
-:1&mu;L disassembled a Degree deodorant stick and soaked in ethanol
+:1)  disassembled a Degree deodorant stick and soaked in ethanol
-:2&mu;L covered the raising platform with Parafilm
+:2)  removed the raising platform and covered it with Parafilm
-:3&mu;L coated the insides of the tube with mineral oil
+:3)  coated the insides of the tube with mineral oil
-:5&mu;L removed platform and poured LB agar into the base of the stick, waited until it solidified
+:5)  removed platform and poured LB agar into the base of the stick, waited until it solidified
-:6&mu;L the Parafilmed platform was put back into the stick and lowered maximally
+:6)  the Parafilmed platform was put back into the stick and lowered maximally
-:7&mu;L LB agar was poured on top of the platform, until the tube was full, waited until it solidifed
+:7)  LB agar was poured on top of the platform, until the tube was full, waited until it solidifed
-:8&mu;L The top of the LB agar was sliced off with an ethanol-sterilized knife
+:8)  The top of the LB agar was sliced off with an ethanol-sterilized knife
-:9&mu;L 400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate
+:9)  400ul of transformed competent cells were plated on the stick as if it was a standard LB agar plate
-:10&mu;L L.B.O. stick was incubated overnight at 37C
+:10)  L.B.O. stick was incubated overnight at 37C