Bioluminescence

Emily's Section

• Metridia luciferase - requires CBP Protein
  • Metridia luciferase link - http://www.ncbi.nlm.nih.gov/pubmed/18754048
  • CBP link - http://www.ncbi.nlm.nih.gov/pubmed/18264586
  • CBP amino acid sequence http://www.rsc.org/ej/PP/2008/b713109g.pdf
  • Oxyluciferin reduction - http://www.ncbi.nlm.nih.gov/pubmed/19872330
  • Luciferin Regenerating Enzyme (LRE) - [http://www.ncbi.nlm.nih.gov/pubmed/11457857 LRE] (in Photinus pyralis)
  • Regeneration of Aequorin in Aequorea Aequorea jellyfish - http://www.nature.com/nature/journal/v256/n5514/pdf/256236a0.pdf
  • Firefly luciferin reaction - http://pubs.acs.org/doi/pdf/10.1021/ja00529a051
  • Light Emission Quanta - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WB5-4DXBGFJ-D5-1&_cdi=6701&_user=1495569&_pii=0003986160902083&_orig=search&_coverDate=05%2F31%2F1960&_sk=999119998&view=c&wchp=dGLbVzz-zSkzV&md5=f1b9d348749d1304c05f85d9290e3b90&ie=/sdarticle.pdf Spectral Emission and Quantum Yield of Firefly Bioluminescence]
    • One light quantum emitted for every luciferin molecule oxidized
  • CoA stimulation - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B73G9-47G3TGV-1S9-1&_cdi=11521&_user=1495569&_pii=0006300258903810&_orig=search&_coverDate=12%2F31%2F1958&_sk=999729999&view=c&wchp=dGLbVzb-zSkzV&md5=4420b38ef24aba8ca23e0b8ce2b3a953&ie=/sdarticle.pdf CoA in Luminescence]
  • Bacterial luciferase reaction - [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC357244/?page=1 Bacterial Luciferin]
  • Beetle luciferin - [http://www3.interscience.wiley.com/cgi-bin/fulltext/107615738/PDFSTART Putative Beetle Luciferin Synthesis]
  • V. Fischeri Plasmid - [http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WSN-4C7WG76-M-1&_cdi=7051&_user=1495569&_pii=0092867483900636&_orig=search&_coverDate=03%2F31%2F1983&_sk=999679996&view=c&wchp=dGLzVlz-zSkWb&md5=97c3a918d59cbd913da5c00b6805deb9&ie=/sdarticle.pdf V. Fischeri Plasmid]
  • KCal/Mol luciferin in Lightning Bugs - [http://www.springerlink.com/content/w184700gj8767ww7/fulltext.pdf Luciferin KCal/Mol]
  • Spectral Emissions of different species - [http://jgp.rupress.org/content/48/1/95.full.pdf+html Different Species Spectra]

Theo's section

• Self contained part for Photorhabdus luminescens lux operon: http://partsregistry.org/Part:BBa_J70008:Design
• Firefly diagram from Emily's paper:

• Fireflow luciferin normally emits at 560nm but at higher temperatures at 630nm
• http://pubs.acs.org/doi/pdf/10.1021/bi00702a015 Kinetics
• Mg2+ required
• A nice background summary: http://irishmarinelife.com/bioluminescence-symbioses/
• The guy who made the biobrick's logbook: http://openwetware.org/wiki/User:Spenser_Skates/Notebook/pTKX_luciferase_biobrick/2008/04/14

Vibrio

• luxC = acyl coA reductase

Firefly

• The awesome paper http://www.springerlink.com/content/67w1q80435537747/fulltext.pdf

Bill's section

• [http://sciencelinks.jp/j-east/article/200514/000020051405A0424228.php Conversion of normal luciferin into firefly luciferin]
• Don't use luciferinol, it r teh suckage
• [http://jeb.biologists.org/cgi/reprint/137/1/39.pdf Glowing fish what?]

Will's section

• [http://www.ncbi.nlm.nih.gov/pubmed/15162459 Kinetic analysis and modeling of firefly luciferase as a quantitative reporter gene in live mammalian cells.]

• [http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WB5-4DXBGFJ-D5&_user=1495569&_coverDate=05%2F31%2F1960&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=8d7118ece7966e711eb16425e94285cf "Spectral emission and quantum yield of firefly bioluminescence" One rxn=>one photon]

Team:Cambridge/emissionEstimationNotes General Notes for emission estimation

Definition: Quantum Yield (Q)

Anja's section

• [http://www.ncbi.nlm.nih.gov/pubmed/17540326 Paper on mutant luciferase with increased activity]
• Wavelength= 469 nm
• Low leakage rate
• Does not disrupt cell functions/ devt.
• Requires continual addition of luciferin

Peter's section

• [http://arjournals.annualreviews.org/doi/pdf/10.1146/annurev.mi.42.100188.001055?cookieSet=1 Paper on various bacterial luciferase and luciferin synthesis pathways]

Ben's section

• Flashlight fish symbiont luciferase genes have been sequenced but cannot be sucessfully expressed in E. Coli
• Suggested application http://www.yankodesign.com/2010/05/28/illuminate-your-influenza/
• The firefly luciferin regenerating enzyme is patented http://www.freepatentsonline.com/EP0825257.html
• Firefly luciferin biosynthesis appears not to be known. Vibrio Ficheri Lux operon is known. Would VF luciferin work with other luciferases? http://www3.interscience.wiley.com/cgi-bin/fulltext/89014035/PDFSTART

Paul's section

• Precursor to luciferin: http://www.sigmaaldrich.com/catalog/ProductDetail.do?lang=en&N4=261858|ALDRICH&N5=SEARCH_CONCAT_PNO|BRAND_KEY&F=SPEC